Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Joseph V. Raimondo; Uwe Heinemann; Marco de Curtis; Howard P. Goodkin; Chris G. Dulla; Damir Janigro; Akio Ikeda; Chou Ching K. Lin; Premysl Jiruska; Aristea S. Galanopoulou; Christophe Bernard

doi:10.1111/epi.13901

Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Joseph V. Raimondo
, Uwe Heinemann
, Marco de Curtis
, Howard P. Goodkin
, Chris G. Dulla
, Damir Janigro
, Akio Ikeda
, Chou Ching K. Lin
, Premysl Jiruska
, Aristea S. Galanopoulou
, Christophe Bernard

Department of Neurology

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

Original language	English
Pages (from-to)	40-52
Number of pages	13
Journal	Epilepsia
Volume	58
DOIs	https://doi.org/10.1111/epi.13901
Publication status	Published - 2017 Nov

All Science Journal Classification (ASJC) codes

Neurology
Clinical Neurology

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10.1111/epi.13901

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Raimondo, J. V., Heinemann, U., de Curtis, M., Goodkin, H. P., Dulla, C. G., Janigro, D., Ikeda, A., Lin, C. C. K., Jiruska, P., Galanopoulou, A. S., & Bernard, C. (2017). Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE. Epilepsia, 58, 40-52. https://doi.org/10.1111/epi.13901

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title = "Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE",

abstract = "In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.",

author = "Raimondo, \{Joseph V.\} and Uwe Heinemann and \{de Curtis\}, Marco and Goodkin, \{Howard P.\} and Dulla, \{Chris G.\} and Damir Janigro and Akio Ikeda and Lin, \{Chou Ching K.\} and Premysl Jiruska and Galanopoulou, \{Aristea S.\} and Christophe Bernard",

note = "Publisher Copyright: Wiley Periodicals, Inc. {\textcopyright} 2017 International League Against Epilepsy",

year = "2017",

month = nov,

doi = "10.1111/epi.13901",

language = "English",

volume = "58",

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AU - Raimondo, Joseph V.

AU - Heinemann, Uwe

AU - de Curtis, Marco

AU - Goodkin, Howard P.

AU - Dulla, Chris G.

AU - Janigro, Damir

AU - Ikeda, Akio

AU - Lin, Chou Ching K.

AU - Jiruska, Premysl

AU - Galanopoulou, Aristea S.

AU - Bernard, Christophe

PY - 2017/11

Y1 - 2017/11

N2 - In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

AB - In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

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JF - Epilepsia

ER -

Methodological standards for in vitro models of epilepsy and epileptic seizures. A TASK1-WG4 report of the AES/ILAE Translational Task Force of the ILAE

Abstract

All Science Journal Classification (ASJC) codes

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