Methods for rapid screening and isolation of bacteria producing acidic lipase

Feasibility studies and novel activity assay protocols

Chien Hung Liu, Wen Ming Chen, Jo-Shu Chang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Acidic lipase finds its commercial values in medical applications and bioremediation of food wastes. In this work, approaches for rapid screening of lipase-producing bacteria were developed and the feasibility assessment of the screening methods was performed. From food waste samples, the proposed screening procedures allowed isolation of sixteen pure bacterial strains expressing higher lipase activity at acidic pH (pH 6.0) than at alkaline pH (pH 9.0). To enhance the accuracy of lipase activity determination under acidic conditions, a novel assay procedure was also developed by deactivating lipase activity by microwave treatment prior to back titration. This additional step could minimize interferences arising from residual lipase activity during conventional direct back-titration methods in measuring lipase activity at acidic pH. Using the four strategies proposed in this work, the best acidic-lipase-producing isolate was obtained by strategy C (SSC) and was identified as Aeromonas sp. C14, displaying an optimal lipase activity of 0.7 U/ml at an acidic pH of 6.0.

Original languageEnglish
Pages (from-to)633-640
Number of pages8
JournalWorld Journal of Microbiology and Biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2007 May 1

Fingerprint

Feasibility Studies
Lipase
Bacteria
Aeromonas
Food
Environmental Biodegradation
Microwaves

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "Acidic lipase finds its commercial values in medical applications and bioremediation of food wastes. In this work, approaches for rapid screening of lipase-producing bacteria were developed and the feasibility assessment of the screening methods was performed. From food waste samples, the proposed screening procedures allowed isolation of sixteen pure bacterial strains expressing higher lipase activity at acidic pH (pH 6.0) than at alkaline pH (pH 9.0). To enhance the accuracy of lipase activity determination under acidic conditions, a novel assay procedure was also developed by deactivating lipase activity by microwave treatment prior to back titration. This additional step could minimize interferences arising from residual lipase activity during conventional direct back-titration methods in measuring lipase activity at acidic pH. Using the four strategies proposed in this work, the best acidic-lipase-producing isolate was obtained by strategy C (SSC) and was identified as Aeromonas sp. C14, displaying an optimal lipase activity of 0.7 U/ml at an acidic pH of 6.0.",
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Methods for rapid screening and isolation of bacteria producing acidic lipase : Feasibility studies and novel activity assay protocols. / Liu, Chien Hung; Chen, Wen Ming; Chang, Jo-Shu.

In: World Journal of Microbiology and Biotechnology, Vol. 23, No. 5, 01.05.2007, p. 633-640.

Research output: Contribution to journalArticle

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