TY - JOUR
T1 - Micro capillary electrophoresis chips integrated with buried SU-8/SOG optical waveguides for bio-analytical applications
AU - Lin, Che Hsin
AU - Lee, Gwo Bin
AU - Chen, Shu Hui
AU - Chang, Guan Liang
N1 - Funding Information:
The author would like to thank to Mr. Chun-Che Lin, Department of Chemistry, NCKU, for his kind help in experimental setup and valuable discussion. We also thank the National Science Council in Taiwan for financial support of the project (NSC 90-2323-B006-011).
PY - 2003/10/15
Y1 - 2003/10/15
N2 - This paper reports an innovative micro electrophoresis chip, which is integrated with buried optical waveguides on glass substrates for on-line detection of bio-analytical samples. A novel buried optical waveguide structure using SU-8/SOG (spin-on-glass) double layers is demonstrated, which can increase light guiding efficiency due to large difference of refractive index between SU-8 (n = 1.8, after hard-baking) and organic-based SOG (n = 1.36). Etched optic fibers are directly inserted into the waveguide channel for connection of light signal between microfluidic devices and peripheral optical sensors. With these novel approaches, delicate optical systems and tedious alignment procedures are not required for biomedical sample detection, resulting in a more compact or even a portable detection system. Experimental results show the developed micro capillary chip can detect Rhodamine B fluoresceins with a concentration as low as 10-7 M by using multi-mode optic fibers as the connection of light between buried waveguides and the optical sensor. Two samples have been demonstrated to verify the performance of the chip, including a mixture of Rhodamine B and Cy 3 fluorescent dyes and FITC-labeled polypeptide samples. Both of them can be separated and detected successfully using the proposed device. The proposed device provides a cheap and simple method for on-line detection of biological samples.
AB - This paper reports an innovative micro electrophoresis chip, which is integrated with buried optical waveguides on glass substrates for on-line detection of bio-analytical samples. A novel buried optical waveguide structure using SU-8/SOG (spin-on-glass) double layers is demonstrated, which can increase light guiding efficiency due to large difference of refractive index between SU-8 (n = 1.8, after hard-baking) and organic-based SOG (n = 1.36). Etched optic fibers are directly inserted into the waveguide channel for connection of light signal between microfluidic devices and peripheral optical sensors. With these novel approaches, delicate optical systems and tedious alignment procedures are not required for biomedical sample detection, resulting in a more compact or even a portable detection system. Experimental results show the developed micro capillary chip can detect Rhodamine B fluoresceins with a concentration as low as 10-7 M by using multi-mode optic fibers as the connection of light between buried waveguides and the optical sensor. Two samples have been demonstrated to verify the performance of the chip, including a mixture of Rhodamine B and Cy 3 fluorescent dyes and FITC-labeled polypeptide samples. Both of them can be separated and detected successfully using the proposed device. The proposed device provides a cheap and simple method for on-line detection of biological samples.
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U2 - 10.1016/S0924-4247(03)00351-0
DO - 10.1016/S0924-4247(03)00351-0
M3 - Article
AN - SCOPUS:0142059976
SN - 0924-4247
VL - 107
SP - 125
EP - 131
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
IS - 2
ER -