Microalgae harvesting and subsequent biodiesel conversion

Dang Thuan Tran, Bich Hanh Le, Duu Jong Lee, Ching Lung Chen, Hsiang Yu Wang, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Chlorella vulgaris ESP-31 containing 22.7% lipid was harvested by coagulation (using chitosan and polyaluminium chloride (PACl) as the coagulants) and centrifugation. The harvested ESP-31 was directly employed as the oil source for biodiesel production via transesterification catalyzed by immobilized Burkholderia lipase and by a synthesized solid catalyst (SrO/SiO2). Both enzymatic and chemical transesterification were significantly inhibited in the presence of PACl, while the immobilized lipase worked well with wet chitosan-coagulated ESP-31, giving a high biodiesel conversion of 97.6% w/w oil, which is at a level comparable to that of biodiesel conversion from centrifugation-harvested microalgae (97.1% w/w oil). The immobilized lipase can be repeatedly used for three cycles without significant loss of its activity. The solid catalyst SrO/SiO2 worked well with water-removed centrifuged ESP-31 with a biodiesel conversion of 80% w/w oil, but the conversion became lower (55.7-61.4% w/w oil) when using water-removed chitosan-coagulated ESP-31 as the oil source.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalBioresource technology
Publication statusPublished - 2013 Jul

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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