Microarray profiling of gene expression patterns in bladder tumor cells treated with genistein

Chin Chuan Chen, Biehuoy Shieh, Ying Tai Jin, Yun Er Liau, Chia Hui Huang, Ji Tzung Liou, Li Wha Wu, Wenya Huang, Kung Chia Young, Ming Derg Lai, Hsiao Sheng Liu, Ching Li

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Microarray technology was used to gain an insight into the molecular events of tumor cell growth inhibition mediated by the soy isoflavone genistein. For this, a susceptible bladder tumor line TCCSUP was treated with the inhibitory dose “50 μM” of genistein for various periods of time, followed by mRNA isolations, cDNA probe preparations, and hybridization individually to cDNA chips containing 884 sequence-verified known human genes. After analyzing the hybridization signals with a simple quantitative method developed by this study, we detected that egr-1, whose expression has been associated with proliferation and differentiation, was transiently induced and this expression pattern was later confirmed by RT-PCR. Thus, microarray technology is a reliable and powerful tool for profiling gene expression patterns in many biological systems related to cancer. We further detected many groups of genes with distinct expression profiles and most of them encode for proteins that regulate the signal transduction or the cell cycle pathways. These genes warrant further investigation as regards their roles in the susceptibility of the tumor cell line to the antitumor drug.

Original languageEnglish
Pages (from-to)214-222
Number of pages9
JournalJournal of biomedical science
Volume8
Issue number2
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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