Microbial ecology and performance of ammonia oxidizing bacteria (AOB) in biological processes treating petrochemical wastewater with high strength of ammonia

Effect of Na2CO3 addition

Liang-Ming Whang, K. H. Yang, Y. F. Yang, Y. L. Han, Y. J. Chen, S. S. Cheng

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This study evaluated nitrification performance and microbial ecology of AOB in a full-scale biological process, powder activated carbon treatment (PACT), and a pilot-scale biological process, moving bed biofilm reactor (MBBR), treating wastewater collected from a petrochemical industry park. The petrochemical influent wastewater characteristics showed a relative low carbon to nitrogen ratio around 1 with average COD and ammonia concentrations of 310mg/L and 325 mg-N/L, respectively. The average nitrification efficiency of the full-scale PACT process was around 11% during this study. For the pilot-scale MBBR, the average nitrification efficiency was 24% during the Run I operation mode, which provided a slightly better performance in nitrification than that of the PACT process. During the Run II operation, the pH control mode was switched from addition of NaOH to Na2CO3, leading to a significant improvement in nitrification efficiency of 51%. In addition to a dramatic change in nitrification performance, the microbial ecology of AOB, monitored with the terminal restriction fragment length polymorphism (T-RFLP) molecular methodology, was found to be different between Runs I and II. The amoA-based TRFLP results indicated that Nitrosomonas europaea lineage was the dominant AOB population during Run l operation, while Nitrosospira - like AOB was dominant during Run II operation. To confirm the effects of Na 2CO3 addition on the nitrification performance and AOB microbial ecology observed in the MBBR process, batch experiments were conducted. The results suggest that addition of Na2CO3 as a pH control strategy can improve nitrification performance and also influence AOB microbial ecology as well. Although the exact mechanisms are not clear at this time, the results showing the effects of adding different buffering chemicals such as NaOH or Na2CO3 on AOB populations have never been demonstrated until this study.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalWater Science and Technology
Volume59
Issue number2
DOIs
Publication statusPublished - 2009 Mar 2

Fingerprint

microbial ecology
Nitrification
Ecology
Petrochemicals
biological processes
nitrification
Ammonia
Bacteria
Wastewater
ammonia
wastewater
bacterium
Activated carbon treatment
Biofilms
activated carbon
biofilm
Powders
petrochemical industry
effect
petrochemical

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Water Science and Technology

Cite this

@article{444d20f7eb574328b2f267d2d02a643c,
title = "Microbial ecology and performance of ammonia oxidizing bacteria (AOB) in biological processes treating petrochemical wastewater with high strength of ammonia: Effect of Na2CO3 addition",
abstract = "This study evaluated nitrification performance and microbial ecology of AOB in a full-scale biological process, powder activated carbon treatment (PACT), and a pilot-scale biological process, moving bed biofilm reactor (MBBR), treating wastewater collected from a petrochemical industry park. The petrochemical influent wastewater characteristics showed a relative low carbon to nitrogen ratio around 1 with average COD and ammonia concentrations of 310mg/L and 325 mg-N/L, respectively. The average nitrification efficiency of the full-scale PACT process was around 11{\%} during this study. For the pilot-scale MBBR, the average nitrification efficiency was 24{\%} during the Run I operation mode, which provided a slightly better performance in nitrification than that of the PACT process. During the Run II operation, the pH control mode was switched from addition of NaOH to Na2CO3, leading to a significant improvement in nitrification efficiency of 51{\%}. In addition to a dramatic change in nitrification performance, the microbial ecology of AOB, monitored with the terminal restriction fragment length polymorphism (T-RFLP) molecular methodology, was found to be different between Runs I and II. The amoA-based TRFLP results indicated that Nitrosomonas europaea lineage was the dominant AOB population during Run l operation, while Nitrosospira - like AOB was dominant during Run II operation. To confirm the effects of Na 2CO3 addition on the nitrification performance and AOB microbial ecology observed in the MBBR process, batch experiments were conducted. The results suggest that addition of Na2CO3 as a pH control strategy can improve nitrification performance and also influence AOB microbial ecology as well. Although the exact mechanisms are not clear at this time, the results showing the effects of adding different buffering chemicals such as NaOH or Na2CO3 on AOB populations have never been demonstrated until this study.",
author = "Liang-Ming Whang and Yang, {K. H.} and Yang, {Y. F.} and Han, {Y. L.} and Chen, {Y. J.} and Cheng, {S. S.}",
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Microbial ecology and performance of ammonia oxidizing bacteria (AOB) in biological processes treating petrochemical wastewater with high strength of ammonia : Effect of Na2CO3 addition. / Whang, Liang-Ming; Yang, K. H.; Yang, Y. F.; Han, Y. L.; Chen, Y. J.; Cheng, S. S.

In: Water Science and Technology, Vol. 59, No. 2, 02.03.2009, p. 223-231.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microbial ecology and performance of ammonia oxidizing bacteria (AOB) in biological processes treating petrochemical wastewater with high strength of ammonia

T2 - Effect of Na2CO3 addition

AU - Whang, Liang-Ming

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AU - Cheng, S. S.

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