Microbial volatile compounds-induced cytotoxicity in the yeast Saccharomyces cerevisiae: The role of MAPK signaling and proteasome regulatory pathway

Pei Hsuan Wu, Yueh Lin Ho, Tzong-Shiann Ho, Ching Han Chang, Je Chiuan Ye, Ching Han Wang, Huang-Mo Sung, Hao-Jen Huang, Ching-Chuan Liu

Research output: Contribution to journalArticle

Abstract

Microbial volatile organic compounds (mVCs) are formed in the metabolism of microorganisms and widely distributed in nature and pose threats to human health. However, the air pollution by microorganisms is a situation which is poorly understood. In this study, the cytotoxicity of E. aerogenes VCs was evaluated in the model organism Saccharomyces cerevisiae. E. aerogenes VCs inhibited the survival of yeast and triggered the formation of intracellular reactive oxygen species (ROS). The hypersensitive of MAP kinase mpk1/slt2 and 19S regulatory assembly chaperone adc17 mutants to the E. aerogenes VCs indicated cell wall integrity (CWI) pathway together with stress-inducible proteasome assembly regulation are essentially involved in mVCs tolerance mechanism. Furthermore, exposure to the mVCs resulted in the transcriptional upregulation of the CWI pathway, the regulatory particle assembly chaperones, and genes involved in proteasome regulations. Our research suggested that the ROS/MAPK signaling and proteasome regulatory pathway play pivotal roles in the integration and fine-tuning of the mVCs stress response. This study provides a molecular framework for future study of the effects of mVCs on more complex organisms, such as humans.

Original languageEnglish
Pages (from-to)786-795
Number of pages10
JournalChemosphere
Volume233
DOIs
Publication statusPublished - 2019 Oct 1

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Volatile Organic Compounds
Proteasome Endopeptidase Complex
Cytotoxicity
Volatile organic compounds
Yeast
volatile organic compound
yeast
Saccharomyces cerevisiae
Yeasts
Microorganisms
Cell Wall
Reactive Oxygen Species
microorganism
Oxygen
Air Pollution
Air pollution
Metabolism
Phosphotransferases
Up-Regulation
atmospheric pollution

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Microbial volatile compounds-induced cytotoxicity in the yeast Saccharomyces cerevisiae: The role of MAPK signaling and proteasome regulatory pathway",
abstract = "Microbial volatile organic compounds (mVCs) are formed in the metabolism of microorganisms and widely distributed in nature and pose threats to human health. However, the air pollution by microorganisms is a situation which is poorly understood. In this study, the cytotoxicity of E. aerogenes VCs was evaluated in the model organism Saccharomyces cerevisiae. E. aerogenes VCs inhibited the survival of yeast and triggered the formation of intracellular reactive oxygen species (ROS). The hypersensitive of MAP kinase mpk1/slt2 and 19S regulatory assembly chaperone adc17 mutants to the E. aerogenes VCs indicated cell wall integrity (CWI) pathway together with stress-inducible proteasome assembly regulation are essentially involved in mVCs tolerance mechanism. Furthermore, exposure to the mVCs resulted in the transcriptional upregulation of the CWI pathway, the regulatory particle assembly chaperones, and genes involved in proteasome regulations. Our research suggested that the ROS/MAPK signaling and proteasome regulatory pathway play pivotal roles in the integration and fine-tuning of the mVCs stress response. This study provides a molecular framework for future study of the effects of mVCs on more complex organisms, such as humans.",
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Microbial volatile compounds-induced cytotoxicity in the yeast Saccharomyces cerevisiae : The role of MAPK signaling and proteasome regulatory pathway. / Wu, Pei Hsuan; Ho, Yueh Lin; Ho, Tzong-Shiann; Chang, Ching Han; Ye, Je Chiuan; Wang, Ching Han; Sung, Huang-Mo; Huang, Hao-Jen; Liu, Ching-Chuan.

In: Chemosphere, Vol. 233, 01.10.2019, p. 786-795.

Research output: Contribution to journalArticle

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AU - Wu, Pei Hsuan

AU - Ho, Yueh Lin

AU - Ho, Tzong-Shiann

AU - Chang, Ching Han

AU - Ye, Je Chiuan

AU - Wang, Ching Han

AU - Sung, Huang-Mo

AU - Huang, Hao-Jen

AU - Liu, Ching-Chuan

PY - 2019/10/1

Y1 - 2019/10/1

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