Microchip electrophoresis and related technologies

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microchip electrophoresis is emerging as a highly promising method for fast analysis with minimum amount of analytes. While this platform has been well recognized, there are many technical challenges remained to be solved in order to broaden its usefulness. In the first part of this presentation, we will demonstrate on-chip assays for fast analysis of estrogen related binding cascade to monitor both ligand binding and estrogen responsive element (ERE) binding, which serve as screening assays for steroid-like compounds. The method requires only nano-grams of samples and the run time is within minutes. In the second part of this presentation, I will demonstrate a novel surface modification for a common substrate of microchip electrophoresis, Polydimethylsiloxane (PDMS). We have created a permanently hydrophilic PDMS surfaces using electrostatic self-assembly and chemical cross-linking. Electrokinetic analysis of the coatings in microchannels always exposed to air gave constant electroosmotic flow during the six months that the measurements were done.

Original languageEnglish
Title of host publicationEmerging Information Technology Conference 2005
Pages161-164
Number of pages4
DOIs
Publication statusPublished - 2005 Dec 1
EventEmerging Information Technology Conference 2005 - Taipei, Taiwan
Duration: 2005 Aug 152005 Aug 16

Publication series

NameEmerging Information Technology Conference 2005
Volume2005

Other

OtherEmerging Information Technology Conference 2005
CountryTaiwan
CityTaipei
Period05-08-1505-08-16

Fingerprint

Polydimethylsiloxane
Electrophoresis
Assays
Microchannels
Self assembly
Surface treatment
Electrostatics
Screening
Ligands
Coatings
Substrates
Air
Estrogens

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Chen, S-H. (2005). Microchip electrophoresis and related technologies. In Emerging Information Technology Conference 2005 (pp. 161-164). [1544375] (Emerging Information Technology Conference 2005; Vol. 2005). https://doi.org/10.1109/EITC.2005.1544375
Chen, Shu-Hui. / Microchip electrophoresis and related technologies. Emerging Information Technology Conference 2005. 2005. pp. 161-164 (Emerging Information Technology Conference 2005).
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Chen, S-H 2005, Microchip electrophoresis and related technologies. in Emerging Information Technology Conference 2005., 1544375, Emerging Information Technology Conference 2005, vol. 2005, pp. 161-164, Emerging Information Technology Conference 2005, Taipei, Taiwan, 05-08-15. https://doi.org/10.1109/EITC.2005.1544375

Microchip electrophoresis and related technologies. / Chen, Shu-Hui.

Emerging Information Technology Conference 2005. 2005. p. 161-164 1544375 (Emerging Information Technology Conference 2005; Vol. 2005).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen S-H. Microchip electrophoresis and related technologies. In Emerging Information Technology Conference 2005. 2005. p. 161-164. 1544375. (Emerging Information Technology Conference 2005). https://doi.org/10.1109/EITC.2005.1544375