TY - JOUR
T1 - Microfluidic aptasensor POC device for determination of whole blood potassium
AU - Tseng, Chin Chung
AU - Lu, Song Yu
AU - Chen, Szu Jui
AU - Wang, Ju Ming
AU - Fu, Lung Ming
AU - Wu, Yi Hong
N1 - Funding Information:
The authors would like to thank the Ministry of Science and Technology of Taiwan for the financial support of this study under Grant Nos. MOST 106-2314-B-006-085 -MY3 , MOST 106-2221-E-006-253-MY3 , MOST 107-2622-B-006-007-CC2 , and MOST 106TFDA-A-103 .
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/4/22
Y1 - 2022/4/22
N2 - An integrated microfluidic Au nanoparticle (AuNP) aptasensor device is proposed for monitoring the concentration of potassium (K+) ions in the bloodstream of patients with chronic kidney disease (CKD). In the proposed detection device, the AuNPs in the AuNP/aptamer complex are displaced by the serum K+ ions and react with NaCl to produce a color change in the detection region from which the K+ ion concentration is then inversely derived. The microfluidic device comprises two main components, namely an AuNP aptasensor PMMA (Poly(methyl methacrylate))/paper-microchip and a colorimetric analysis system for the quantitative detection of K+ ion concentration in whole blood. The functions of PMMA/paper microchips include reagent storage, K+ ion/aptamer reaction, and separation of serum from whole blood samples (blood filter). Experimental results show that the microfluidic device provides a linear response over the K+ ion concentration in range of 0.05–9 mM in artificial serum and has a detection limit (LOD) of 0.01 mM. Moreover, the detection results obtained for the 137 whole blood and 287 serum samples of CKD patients are very consistent (R2 = 0.968 and R2 = 0.980) with the measurement results obtained using an ion-selective electrodes (ISE) method. Results confirm that the current microfluidic aptasensor device provides a highly-sensitive and convenient method for performing the point-of-care (POC) monitoring of the whole blood K+ ion concentration.
AB - An integrated microfluidic Au nanoparticle (AuNP) aptasensor device is proposed for monitoring the concentration of potassium (K+) ions in the bloodstream of patients with chronic kidney disease (CKD). In the proposed detection device, the AuNPs in the AuNP/aptamer complex are displaced by the serum K+ ions and react with NaCl to produce a color change in the detection region from which the K+ ion concentration is then inversely derived. The microfluidic device comprises two main components, namely an AuNP aptasensor PMMA (Poly(methyl methacrylate))/paper-microchip and a colorimetric analysis system for the quantitative detection of K+ ion concentration in whole blood. The functions of PMMA/paper microchips include reagent storage, K+ ion/aptamer reaction, and separation of serum from whole blood samples (blood filter). Experimental results show that the microfluidic device provides a linear response over the K+ ion concentration in range of 0.05–9 mM in artificial serum and has a detection limit (LOD) of 0.01 mM. Moreover, the detection results obtained for the 137 whole blood and 287 serum samples of CKD patients are very consistent (R2 = 0.968 and R2 = 0.980) with the measurement results obtained using an ion-selective electrodes (ISE) method. Results confirm that the current microfluidic aptasensor device provides a highly-sensitive and convenient method for performing the point-of-care (POC) monitoring of the whole blood K+ ion concentration.
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U2 - 10.1016/j.aca.2022.339722
DO - 10.1016/j.aca.2022.339722
M3 - Article
C2 - 35361435
AN - SCOPUS:85126513763
SN - 0003-2670
VL - 1203
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
M1 - 339722
ER -