Microfluidic chip for high efficiency DNA extraction

Yung Chiang Chung, Ming Shiung Jan, Yu Cheng Lin, Ju Hwa Lin, Wang Chin Cheng, Chia Yu Fan

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

A high efficiency DNA extraction microchip was designed to extract DNA from lysed cells using immobilized beads and the solution flowing back and forth. This chip was able to increase the extraction efficiency by 2-fold when there was no serum. When serum existed in the solution, the extraction efficiency of immobilized beads was 88-fold higher than that of free beads. The extraction efficiency of the microchip was tested under different conditions and numbers of E. coli cells. When the number of E. coli cells was between 106 and 108 in 25 μl of whole blood, the extraction efficiency using immobilized beads was only slightly higher than that using free beads (100 to 101 fold). When the number of E. coli cells was in the range 104 to 106 in 25 μl of whole blood, the extraction efficiency of immobilized beads was greater than that of the free beads (101 to 102 fold). When the number of E. coli cells was lower, in the range 103 to 104 in 25 μl of whole blood, the extraction efficiency of immobilized beads was much higher than that of the free beads (102 to 103 fold). This study indicated that DNA could be efficiently extracted even when the number of bacterial cells was smaller (105 to 103). This microfluidic extraction chip could find potential applications in rare sample genomic study.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalLab on a Chip
Volume4
Issue number2
DOIs
Publication statusPublished - 2004 Apr

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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