Microfluidic chip of fast DNA hybridization using denature and motion of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Chaung Di Chueh, Chuan You Ye, Li Wei Lai, Wei Chieh Liao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages960-963
Number of pages4
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Publication series

Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09-01-0509-01-08

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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    Chung, Y. C., Lin, Y-C., Chueh, C. D., Ye, C. Y., Lai, L. W., & Liao, W. C. (2009). Microfluidic chip of fast DNA hybridization using denature and motion of nucleic acids. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 960-963). [5068733] (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068733