Microfluidic chips for DNA amplification, electrophoresis separation and on-line optical detection

Gwo Bin Lee, Che Hsin Lin, Fu Chun Huang, Chia Sheng Liao, Chia Yen Lee, Shu-Hui Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

This paper reports an innovative microfluidic chip capable of performing DNA amplification (Polymerase Chain Reaction, PCR), electrokinetic sample injection and separation, and on-line optical detection of DNA. All microfluidic modules are integrated on cheap and biocompatible soda-lime glass substrates using a simple and reliable fabrication process. With this approach. DNA samples could be first duplicated using the micro PCR module, then injected and separated in the micro electrophoretic channels driven by electrokinetic forces, and finally detected optically by buried optical waveguides downstream the separation channel. In order to have a better separation efficiency for DNA samples, a novel surface treatment method by coating a thin layer of spin-on-glass (SOG) is developed. Experimental data show that DNA samples can be successfully duplicated using the micro PCR device with less sample and reagent volumes in a shorter period thanks to lower thermal inertia of the micro devices. DNA samples can be injected, separated and detected successfully in the subsequent microfluidic channels. The integrated microfluidic device could be crucial for genetic analysis.

Original languageEnglish
Title of host publicationProceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
Pages423-426
Number of pages4
Publication statusPublished - 2003
EventIEEE Sixteenth Annual International Conference on Micro Electro Mechanical Systems - Kyoto, Japan
Duration: 2003 Jan 192003 Jan 23

Other

OtherIEEE Sixteenth Annual International Conference on Micro Electro Mechanical Systems
CountryJapan
CityKyoto
Period03-01-1903-01-23

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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