Microfluidic Enzyme-Linked Immunosorbent Assay Technology

L. James Lee, Shang Tiang Yang, Siyi Lai, Yunling Bai, Wei Cho Huang, Yi-Je Juang

Research output: Chapter in Book/Report/Conference proceedingChapter

28 Citations (Scopus)

Abstract

In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.

Original languageEnglish
Title of host publicationAdvances in Clinical Chemistry
EditorsGregory Makowski
Pages255-295
Number of pages41
DOIs
Publication statusPublished - 2006 Sep 7

Publication series

NameAdvances in Clinical Chemistry
Volume42
ISSN (Print)0065-2423

Fingerprint

Immunosorbents
Microfluidics
Assays
Enzyme-Linked Immunosorbent Assay
Technology
Enzymes
Microtechnology
Food Safety
Environmental Monitoring
Biological Factors
Immunoassay
Food safety
Immobilization
Microfabrication
Biomolecules
Surface treatment
Equipment and Supplies
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Clinical Biochemistry

Cite this

Lee, L. J., Yang, S. T., Lai, S., Bai, Y., Huang, W. C., & Juang, Y-J. (2006). Microfluidic Enzyme-Linked Immunosorbent Assay Technology. In G. Makowski (Ed.), Advances in Clinical Chemistry (pp. 255-295). (Advances in Clinical Chemistry; Vol. 42). https://doi.org/10.1016/S0065-2423(06)42007-2
Lee, L. James ; Yang, Shang Tiang ; Lai, Siyi ; Bai, Yunling ; Huang, Wei Cho ; Juang, Yi-Je. / Microfluidic Enzyme-Linked Immunosorbent Assay Technology. Advances in Clinical Chemistry. editor / Gregory Makowski. 2006. pp. 255-295 (Advances in Clinical Chemistry).
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Lee, LJ, Yang, ST, Lai, S, Bai, Y, Huang, WC & Juang, Y-J 2006, Microfluidic Enzyme-Linked Immunosorbent Assay Technology. in G Makowski (ed.), Advances in Clinical Chemistry. Advances in Clinical Chemistry, vol. 42, pp. 255-295. https://doi.org/10.1016/S0065-2423(06)42007-2

Microfluidic Enzyme-Linked Immunosorbent Assay Technology. / Lee, L. James; Yang, Shang Tiang; Lai, Siyi; Bai, Yunling; Huang, Wei Cho; Juang, Yi-Je.

Advances in Clinical Chemistry. ed. / Gregory Makowski. 2006. p. 255-295 (Advances in Clinical Chemistry; Vol. 42).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.

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Lee LJ, Yang ST, Lai S, Bai Y, Huang WC, Juang Y-J. Microfluidic Enzyme-Linked Immunosorbent Assay Technology. In Makowski G, editor, Advances in Clinical Chemistry. 2006. p. 255-295. (Advances in Clinical Chemistry). https://doi.org/10.1016/S0065-2423(06)42007-2