TY - CHAP
T1 - Microfluidic Enzyme-Linked Immunosorbent Assay Technology
AU - Lee, L. James
AU - Yang, Shang Tiang
AU - Lai, Siyi
AU - Bai, Yunling
AU - Huang, Wei Cho
AU - Juang, Yi Je
PY - 2006
Y1 - 2006
N2 - In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.
AB - In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.
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U2 - 10.1016/S0065-2423(06)42007-2
DO - 10.1016/S0065-2423(06)42007-2
M3 - Chapter
C2 - 17131629
AN - SCOPUS:33748150435
SN - 0120103427
SN - 9780120103423
T3 - Advances in Clinical Chemistry
SP - 255
EP - 295
BT - Advances in Clinical Chemistry
A2 - Makowski, Gregory
ER -