Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Yuan Jhe Chuang, Yi Cheng Tsai, Yi Ling Lo, Wen Bin Lee, Shu Chu Shiesh, Hwan You Chang, Keng Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

Original languageEnglish
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages568-571
Number of pages4
ISBN (Electronic)9781538627310
DOIs
Publication statusPublished - 2017 Jul 26
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan
Duration: 2017 Jun 182017 Jun 22

Publication series

NameTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan
CityKaohsiung
Period17-06-1817-06-22

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

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