Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Yuan Jhe Chuang, Yi Cheng Tsai, Yi Ling Lo, Wen Bin Lee, Shu-Chu Shiesh, Hwan You Chang, Keng-Fu Hsu, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

Original languageEnglish
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages568-571
Number of pages4
ISBN (Electronic)9781538627310
DOIs
Publication statusPublished - 2017 Jul 26
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan
Duration: 2017 Jun 182017 Jun 22

Publication series

NameTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan
CityKaohsiung
Period17-06-1817-06-22

Fingerprint

Bacteriophages
chutes
Microfluidics
reagents
affinity
Screening
screening
platforms
Biopsy
cancer
Display devices
Tissue
Peptides
peptides
Peptide Aptamers
Peptide Nucleic Acids
Nucleic acids
nucleic acids
amino acids
Amino acids

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Hung, L. Y., Fu, C. Y., Wang, C. H., Chuang, Y. J., Tsai, Y. C., Lo, Y. L., ... Lee, G. B. (2017). Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 568-571). [7994112] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994112
Hung, Lien Yu ; Fu, Chien Yu ; Wang, Chih Hung ; Chuang, Yuan Jhe ; Tsai, Yi Cheng ; Lo, Yi Ling ; Lee, Wen Bin ; Shiesh, Shu-Chu ; Chang, Hwan You ; Hsu, Keng-Fu ; Lee, Gwo Bin. / Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 568-571 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).
@inproceedings{27a051f4cf464619bc5491f2a91ee88b,
title = "Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer",
abstract = "This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.",
author = "Hung, {Lien Yu} and Fu, {Chien Yu} and Wang, {Chih Hung} and Chuang, {Yuan Jhe} and Tsai, {Yi Cheng} and Lo, {Yi Ling} and Lee, {Wen Bin} and Shu-Chu Shiesh and Chang, {Hwan You} and Keng-Fu Hsu and Lee, {Gwo Bin}",
year = "2017",
month = "7",
day = "26",
doi = "10.1109/TRANSDUCERS.2017.7994112",
language = "English",
series = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "568--571",
booktitle = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",
address = "United States",

}

Hung, LY, Fu, CY, Wang, CH, Chuang, YJ, Tsai, YC, Lo, YL, Lee, WB, Shiesh, S-C, Chang, HY, Hsu, K-F & Lee, GB 2017, Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994112, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 568-571, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, 17-06-18. https://doi.org/10.1109/TRANSDUCERS.2017.7994112

Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chuang, Yuan Jhe; Tsai, Yi Cheng; Lo, Yi Ling; Lee, Wen Bin; Shiesh, Shu-Chu; Chang, Hwan You; Hsu, Keng-Fu; Lee, Gwo Bin.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 568-571 7994112 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

AU - Hung, Lien Yu

AU - Fu, Chien Yu

AU - Wang, Chih Hung

AU - Chuang, Yuan Jhe

AU - Tsai, Yi Cheng

AU - Lo, Yi Ling

AU - Lee, Wen Bin

AU - Shiesh, Shu-Chu

AU - Chang, Hwan You

AU - Hsu, Keng-Fu

AU - Lee, Gwo Bin

PY - 2017/7/26

Y1 - 2017/7/26

N2 - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

AB - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

UR - http://www.scopus.com/inward/record.url?scp=85029361482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029361482&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2017.7994112

DO - 10.1109/TRANSDUCERS.2017.7994112

M3 - Conference contribution

AN - SCOPUS:85029361482

T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

SP - 568

EP - 571

BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - Institute of Electrical and Electronics Engineers Inc.

ER -

Hung LY, Fu CY, Wang CH, Chuang YJ, Tsai YC, Lo YL et al. Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 568-571. 7994112. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/TRANSDUCERS.2017.7994112