Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples

Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Yuan Jhe Chuang, Yi Cheng Tsai, Yi Ling Lo, Pang Hung Hsu, Hwan You Chang, Shu-Chu Shiesh, Keng-Fu Hsu, Gwo Bin Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. However, the benchtop protocols for both screening technologies are relatively lengthy and require well-trained personnel. We therefore developed a novel, integrated microfluidic system capable of automating SELEX and phage display technology. Instead of using cancer cell lines, it is the first work which used tissue slides as screening targets, which possess more complicated and uncovered information for affinity reagents to recognize. This allowed for the identification of aptamer (nucleic acid) and peptide probes specific to OvCa cells and tissues. Furthermore, this developed system could be readily modified to uncover affinity reagents for diagnostics or even target therapy of other cancer cell types in the future.

Original languageEnglish
Article number054108
JournalBiomicrofluidics
Volume12
Issue number5
DOIs
Publication statusPublished - 2018 Sep 1

Fingerprint

Microfluidics
Early Detection of Cancer
Ovarian Neoplasms
reagents
affinity
Screening
SELEX Aptamer Technique
screening
platforms
cancer
Tissue
Technology
Bacteriophages
Biomarkers
Neoplasms
Display devices
Ligands
Cells
Nucleic Acid Probes
Peptide Nucleic Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Hung, L. Y., Fu, C. Y., Wang, C. H., Chuang, Y. J., Tsai, Y. C., Lo, Y. L., ... Lee, G. B. (2018). Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. Biomicrofluidics, 12(5), [054108]. https://doi.org/10.1063/1.5050451
Hung, Lien Yu ; Fu, Chien Yu ; Wang, Chih Hung ; Chuang, Yuan Jhe ; Tsai, Yi Cheng ; Lo, Yi Ling ; Hsu, Pang Hung ; Chang, Hwan You ; Shiesh, Shu-Chu ; Hsu, Keng-Fu ; Lee, Gwo Bin. / Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. In: Biomicrofluidics. 2018 ; Vol. 12, No. 5.
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Hung, LY, Fu, CY, Wang, CH, Chuang, YJ, Tsai, YC, Lo, YL, Hsu, PH, Chang, HY, Shiesh, S-C, Hsu, K-F & Lee, GB 2018, 'Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples', Biomicrofluidics, vol. 12, no. 5, 054108. https://doi.org/10.1063/1.5050451

Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chuang, Yuan Jhe; Tsai, Yi Cheng; Lo, Yi Ling; Hsu, Pang Hung; Chang, Hwan You; Shiesh, Shu-Chu; Hsu, Keng-Fu; Lee, Gwo Bin.

In: Biomicrofluidics, Vol. 12, No. 5, 054108, 01.09.2018.

Research output: Contribution to journalArticle

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AU - Lo, Yi Ling

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AU - Chang, Hwan You

AU - Shiesh, Shu-Chu

AU - Hsu, Keng-Fu

AU - Lee, Gwo Bin

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