Abstract
Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. However, the benchtop protocols for both screening technologies are relatively lengthy and require well-trained personnel. We therefore developed a novel, integrated microfluidic system capable of automating SELEX and phage display technology. Instead of using cancer cell lines, it is the first work which used tissue slides as screening targets, which possess more complicated and uncovered information for affinity reagents to recognize. This allowed for the identification of aptamer (nucleic acid) and peptide probes specific to OvCa cells and tissues. Furthermore, this developed system could be readily modified to uncover affinity reagents for diagnostics or even target therapy of other cancer cell types in the future.
| Original language | English |
|---|---|
| Article number | 054108 |
| Journal | Biomicrofluidics |
| Volume | 12 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2018 Sep 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Materials Science(all)
- Genetics
- Condensed Matter Physics
- Physical and Theoretical Chemistry
Cite this
}
Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chuang, Yuan Jhe; Tsai, Yi Cheng; Lo, Yi Ling; Hsu, Pang Hung; Chang, Hwan You; Shiesh, Shu-Chu; Hsu, Keng-Fu; Lee, Gwo Bin.
In: Biomicrofluidics, Vol. 12, No. 5, 054108, 01.09.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples
AU - Hung, Lien Yu
AU - Fu, Chien Yu
AU - Wang, Chih Hung
AU - Chuang, Yuan Jhe
AU - Tsai, Yi Cheng
AU - Lo, Yi Ling
AU - Hsu, Pang Hung
AU - Chang, Hwan You
AU - Shiesh, Shu-Chu
AU - Hsu, Keng-Fu
AU - Lee, Gwo Bin
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. However, the benchtop protocols for both screening technologies are relatively lengthy and require well-trained personnel. We therefore developed a novel, integrated microfluidic system capable of automating SELEX and phage display technology. Instead of using cancer cell lines, it is the first work which used tissue slides as screening targets, which possess more complicated and uncovered information for affinity reagents to recognize. This allowed for the identification of aptamer (nucleic acid) and peptide probes specific to OvCa cells and tissues. Furthermore, this developed system could be readily modified to uncover affinity reagents for diagnostics or even target therapy of other cancer cell types in the future.
AB - Cancer is the most serious disease worldwide, and ovarian cancer (OvCa) is the second most common type of gynecological cancer. There is consequently an urgent need for early-stage detection of OvCa, which requires affinity reagent biomarkers for OvCa. Systematic evolution of ligands by exponential enrichment (SELEX) and phage display technology are two powerful technologies for identifying affinity reagent biomarkers. However, the benchtop protocols for both screening technologies are relatively lengthy and require well-trained personnel. We therefore developed a novel, integrated microfluidic system capable of automating SELEX and phage display technology. Instead of using cancer cell lines, it is the first work which used tissue slides as screening targets, which possess more complicated and uncovered information for affinity reagents to recognize. This allowed for the identification of aptamer (nucleic acid) and peptide probes specific to OvCa cells and tissues. Furthermore, this developed system could be readily modified to uncover affinity reagents for diagnostics or even target therapy of other cancer cell types in the future.
UR - http://www.scopus.com/inward/record.url?scp=85054543075&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054543075&partnerID=8YFLogxK
U2 - 10.1063/1.5050451
DO - 10.1063/1.5050451
M3 - Article
AN - SCOPUS:85054543075
VL - 12
JO - Biomicrofluidics
JF - Biomicrofluidics
SN - 1932-1058
IS - 5
M1 - 054108
ER -