Microfluidic system for detection of α-thalassemia-1 deletion using saliva samples

Kang Yi Lien, Chien Ju Liu, Pao Lin Kuo, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

This current study presents a new miniature, integrated system capable of rapid detection of genetic deletion from saliva samples. Several critical modules including a genomic DNA (gDNA) extraction module, a polymerase chain reaction (PCR) module, and an external optical detection module are integrated into the system. Silica-modified magnetic beads are first incubated with saliva in an extraction chamber with a cell lysis solution. This is followed by the collection of released gDNA onto the surface of the microbeads, which is then further purified and concentrated utilizing a magnetic field generated by an on-chip array of microcoils. Then, genetic deletion of human genes can be specifically amplified by the on-chip PCR module and is immediately detected using the optical detection module. Experimental results show that highquality gDNA with an average concentration of 50.45 ng/μL can be extracted from 100 μL of saliva. The detection of a mutated α-globin gene associated with α-thalassemia-1 of southeast Asian (SEA)-type deletion can be completed within less than 1 h. Moreover, the detection limit of the system is found to be 12.00 pg/μL with a high sensitivity up to 90%. Consequently, the proposed saliva-based miniature system can provide a powerful platform for rapid DNA extraction and detection of genetic diseases.

Original languageEnglish
Pages (from-to)4502-4509
Number of pages8
JournalAnalytical chemistry
Volume81
Issue number11
DOIs
Publication statusPublished - 2009 Jun 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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