Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps

Chih Hao Wang, Yi Yu Chen, Chia Sheng Liao, Huei-Huang Lee, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper reports a new micromachined flow-through polymerase chain reaction (PCR) chip for applications of rapid pathogen diagnosis. The PCR chip comprised a micro thermal control module and a microfluidic control module fabricated using MEMS (micro-electro-mechanical-systems) technology. The micro thermal control module was formed with three individual heating and temperature-sensing sections, each modulating a specific temperature for denaturation, annealing and extension process, respectively. The membrane-activated micropumps were used to transport sample fluids through three reaction regions to adjust the time ratio and cycle numbers for PCR. The experimental results showed that S. pneumoniae detection gene (273 bps) could be amplified successfully using the new flow-through PCR chip. The new PCR chip could be promising for rapid clinical diagnosis of DNA-based infectious disease.

Original languageEnglish
Title of host publication19th IEEE International Conference on Micro Electro Mechanical Systems
Pages374-377
Number of pages4
Publication statusPublished - 2006 Oct 24
Event19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey
Duration: 2006 Jan 222006 Jan 26

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2006
ISSN (Print)1084-6999

Other

Other19th IEEE International Conference on Micro Electro Mechanical Systems
Country/TerritoryTurkey
CityIstanbul
Period06-01-2206-01-26

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps'. Together they form a unique fingerprint.

Cite this