MicroRNA-196a/-196b promote cell metastasis via negative regulation of radixin in human gastric cancer

Ming Ming Tsai, Chia Siu Wang, Chung Ying Tsai, Cheng Yi Chen, Hsiang Cheng Chi, Yi Hsin Tseng, Pei Jung Chung, Yang Hsiang Lin, I. Hsiao Chung, Ching Ying Chen, Kwang Huei Lin

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) play an important role to contribute carcinogenesis. The aim of the current study was to identify useful biomarkers from miRNAs. Differential miRNA profiles were analyzed using the miRNA qRT-PCR-based assay. Two of the most upregulated miRNAs were selected and validated. The miR-196a/-196b levels were significantly increased in gastric cancer (GC) tissues (n= 109). Overexpression of miR-196a/-196b was significantly associated with tumor progression and poorer 5-year survival outcomes. Overexpression of miR-196a/-196b enhances GC cell migration and invasion. Further, radixin was identified as a target gene of miR-196a/-196b. Elevated miR-196a/-196b expression in GC cells led to reduced radixin protein levels and vice versa. Notably, an inverse correlation between miR-196a/-196b and radixin mRNA and protein expression was observed in GC tissues with in situ hybridization and immunohistochemistry analyses. Together, miR-196a/-196b inhibitory oligonucleotides or overexpression of the radixin may thus have therapeutic potential in suppressing GC metastasis.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalCancer Letters
Volume351
Issue number2
DOIs
Publication statusPublished - 2014 Sep 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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