Microwell chips for selection of bio-macromolecules that increase the differentiation capacities of mesenchymal stem cells

Shan Hui Hsu, Yu Hui Ni, Yung Chun Lee

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Microwell chips (25mm×25mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (≈105) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.

Original languageEnglish
Pages (from-to)1100-1109
Number of pages10
JournalMacromolecular Bioscience
Volume13
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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