Microwell chips for selection of bio-macromolecules that increase the differentiation capacities of mesenchymal stem cells

Shan Hui Hsu; Yu Hui Ni; Yung Chun Lee

doi:10.1002/mabi.201200472

Microwell chips for selection of bio-macromolecules that increase the differentiation capacities of mesenchymal stem cells

Shan Hui Hsu, Yu Hui Ni, Yung Chun Lee

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Microwell chips (25mm×25mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (≈10⁵) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.

Original language	English
Pages (from-to)	1100-1109
Number of pages	10
Journal	Macromolecular Bioscience
Volume	13
Issue number	8
DOIs	https://doi.org/10.1002/mabi.201200472
Publication status	Published - 2013 Aug

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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abstract = "Microwell chips (25mm×25mm) are fabricated to select proper substrates for growing three-dimensional (3D) spheroids from mesenchymal stem cells (MSCs). Different bio-macromolecules and their combinations are immobilized on the chip by air plasma treatment and by polyelectrolyte interaction. Only a small number of MSCs (≈105) are needed for each chip. The expression level of N-cadherin, a cell-adhesion molecule, is used as an indicator for cell-cell interactions. MSC spheroids expressing the highest N-cadherin level also show the greatest osteogenic potential. The microwell chip may be used as an efficient platform to screen bio-macromolecules that enhance the differentiation potential of MSCs.",

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Microwell chips for selection of bio-macromolecules that increase the differentiation capacities of mesenchymal stem cells

Abstract

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