Migration patterns and cell functions of adipose-derived stromal cells on self-assembled monolayers with different functional groups

Hsiao Feng Chieh, Fong-chin Su, Sheng Che Lin, Meng-Ru Shen, Jiunn-Der Liao

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10 Citations (Scopus)

Abstract

Microenvironments provide cues to stem cells and induce signals to direct their fate. With a view toward further understanding the correlation between surface chemistry and cell functions of stem cells, adiposederived stromal cells (ADSCs) and self-assembled monolayers terminated with four different functional groups (-CH3, -NH2, -COOH and -OH) were used to assess cell adhesion, migration and differentiation potential in short-term incubation. Quantitative time-lapse microscopic analysis revealed that migration speed and patterns were strongly regulated by surface chemistry. ADSCs showed a mesenchymal migration pattern on the -COOH-modified surface. In contrast, cells on the -CH3-modified surface displayed an amoeboid-like migration pattern. Cell-migration speeds on the chemically-modified surfaces followed the sequence (by tail-groups): -CH3>-COOH>-OH>-NH2. After 1 day of incubation, ADSCs showed a round compact shape and adipogenic differentiation potential on the -CH3-modified surface. The round compact shape and extremely different migration pattern of ADSCs on -CH 3 surfaces were attributed to the lower amount of exposed cell-binding domains of adsorbed proteins. ADSCs exhibited spindle-like shape and higher Collagen II expression on the -COOH-modified surface and well-spread morphology and higher Runx2 expression were observed on the -NH2- and -OH-modified surfaces. Surface chemistry presented a strong influence on cell functions of ADSCs, including cell adhesion, migration and mRNA expression in short-term incubation.

Original languageEnglish
Pages (from-to)94-117
Number of pages24
JournalJournal of Biomaterials Science, Polymer Edition
Volume24
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Self assembled monolayers
Stromal Cells
Functional groups
Cell Movement
Surface chemistry
Cell Adhesion
Cell adhesion
Stem cells
Stem Cells
Cues
Cell Differentiation
Collagen
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

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title = "Migration patterns and cell functions of adipose-derived stromal cells on self-assembled monolayers with different functional groups",
abstract = "Microenvironments provide cues to stem cells and induce signals to direct their fate. With a view toward further understanding the correlation between surface chemistry and cell functions of stem cells, adiposederived stromal cells (ADSCs) and self-assembled monolayers terminated with four different functional groups (-CH3, -NH2, -COOH and -OH) were used to assess cell adhesion, migration and differentiation potential in short-term incubation. Quantitative time-lapse microscopic analysis revealed that migration speed and patterns were strongly regulated by surface chemistry. ADSCs showed a mesenchymal migration pattern on the -COOH-modified surface. In contrast, cells on the -CH3-modified surface displayed an amoeboid-like migration pattern. Cell-migration speeds on the chemically-modified surfaces followed the sequence (by tail-groups): -CH3>-COOH>-OH>-NH2. After 1 day of incubation, ADSCs showed a round compact shape and adipogenic differentiation potential on the -CH3-modified surface. The round compact shape and extremely different migration pattern of ADSCs on -CH 3 surfaces were attributed to the lower amount of exposed cell-binding domains of adsorbed proteins. ADSCs exhibited spindle-like shape and higher Collagen II expression on the -COOH-modified surface and well-spread morphology and higher Runx2 expression were observed on the -NH2- and -OH-modified surfaces. Surface chemistry presented a strong influence on cell functions of ADSCs, including cell adhesion, migration and mRNA expression in short-term incubation.",
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AU - Su, Fong-chin

AU - Lin, Sheng Che

AU - Shen, Meng-Ru

AU - Liao, Jiunn-Der

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