Miniature RT-PCR system for diagnosis of RNA-based viruses

Chia Sheng Liao, Gwo Bin Lee, Hsiao Sheng Liu, Tsung Min Hsieh, Ching Hsing Luo

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

This paper presents an innovative portable chip-based RT-PCR system for amplification of specific nucleic acid and detection of RNA-based viruses. The miniature RT-PCR chip is fabricated using MEMS (Micro-electro-mechanical-system) techniques, and comprises a micro temperature control module and a PDMS (polydimethylsiloxane)-based microfluidic control module. The heating and sensing elements of temperature control module are both made of platinum and are located within the reaction chambers in order to generate a rapid and uniform thermal cycling. The microfluidic control module is capable of automating testing process with minimum human intervention. In this paper, the proposed miniature RT-PCR system is used to amplify and detect two RNA-based viruses, namely dengue virus type-2 and enterovirus 71 (EV 71). The experimental data confirm the ability of the system to perform a two-step RT-PCR process. The developed miniature system provides a crucial tool for the diagnosis of RNA-based viruses.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalNucleic acids research
Volume33
Issue number18
DOIs
Publication statusPublished - 2005

All Science Journal Classification (ASJC) codes

  • Genetics

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