Mirror-based broadband scanner with minimized aberration

Jiun Yann Yu, Yu Yi Tzeng, Chen Han Huang, Hsiang Chen Chui, Shi Wei Chu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To obtain specific biochemical information in optical scanning microscopy, labeling technique is routinely required. Instead of the complex and invasive sample preparation procedures, incorporating spectral acquisition, which commonly requires a broadband light source, provides another mechanism to enhance molecular contrast. But most current optical scanning system is lens-based and thus the spectral bandwidth is limited to several hundred nanometers due to anti-reflection coating and chromatic aberration. The spectral range of interest in biological research covers ultraviolet to infrared.For example, the absorption peak of water falls around 3 μm, while most proteins exhibit absorption in the UV-visible regime. For imaging purpose, the transmission window of skin and cerebral tissues fall around 1300 and 1800 nm, respectively. Therefore, to extend the spectral bandwidth of an optical scanning system from visible to mid-infrared, we propose a system composed of metallic coated mirrors. A common issue n such a mirror-based system is aberrations induced by oblique incidence.We propose to compensate astigmatism by exchanging the sagittal and tangential planes of the converging spherical mirrors in the scanning system. With the aid of an optical design software, we build a diffraction-limited broadband scanning system with wavefront flatness better than ?/4 at focal plane. Combined with a mirror-based objective this microscopic system will exhibit full spectral capability and will be useful in microscopic imaging and therapeutic applications.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging II
DOIs
Publication statusPublished - 2009 Jun 12
EventSingle Molecule Spectroscopy and Imaging II - San Jose, CA, United States
Duration: 2009 Jan 242009 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7185
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Imaging II
CountryUnited States
CitySan Jose, CA
Period09-01-2409-01-25

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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