TY - JOUR
T1 - Modification of platinum microelectrode with molecularly imprinted over-oxidized polypyrrole for dopamine measurement in rat striatum
AU - Tsai, Tien Chun
AU - Han, Huan Zhang
AU - Cheng, Ching Ching
AU - Chen, Li Chia
AU - Chang, Hsien Chang
AU - Chen, Jia Jin Jason
N1 - Funding Information:
The authors would like to thank the Department of Medical Laboratory Science and Biotechnology, NCKU, for assisting UV-Vis spectrophotometer. We would also like to thank the National Health Research Institute of Taiwan for financially supporting the work under contract no. NHRI-EX-99-9835EI.
PY - 2012/8
Y1 - 2012/8
N2 - A highly sensitive and selective electrochemical microelectrode using the molecularly imprinted (MIP) electropolymer of over-oxidized polypyrrole (OPPy) was proposed for in vivo detection of dopamine (DA) which process is different from current approaches for in vitro DA detection using MIP-based macroelectrode. The proposed MIP electrosynthesized by our present method under a DA/pyrrole-monomer concentration ratio of 1 mM/10 mM yielded the best results for DA adsorption, reaching 2.6-fold that of similar microelectrodes without imprinting. Differential normal pulse voltammetry techniques was used to evaluate the DA-imprinted OPPy microelectrodes for demonstrating high selectivity for in vitro DA detection under conditions with various competitive electroactive molecules. The validity of the modified OPPy-MIP thin-film microelectrodes was further verified with amperometric i-t measurements, which showed a lower DA levels of detection (10-100 nM) and the low detection limit of 4.5 nM with signal to noise ratio of 3. Good reproducibility with relative standard deviation of 5.45% and long-term stability within two weeks were also observed. The developed OPPy-MIP microelectrode has been successfully applied for acute in vivo DA detection in the striatum of anesthetized rats with 3,4-dihydroxy-l-phenylalanine (l-DOPA) administration, which can be used for future evaluation of novel therapeutic treatments in Parkinsonian rats.
AB - A highly sensitive and selective electrochemical microelectrode using the molecularly imprinted (MIP) electropolymer of over-oxidized polypyrrole (OPPy) was proposed for in vivo detection of dopamine (DA) which process is different from current approaches for in vitro DA detection using MIP-based macroelectrode. The proposed MIP electrosynthesized by our present method under a DA/pyrrole-monomer concentration ratio of 1 mM/10 mM yielded the best results for DA adsorption, reaching 2.6-fold that of similar microelectrodes without imprinting. Differential normal pulse voltammetry techniques was used to evaluate the DA-imprinted OPPy microelectrodes for demonstrating high selectivity for in vitro DA detection under conditions with various competitive electroactive molecules. The validity of the modified OPPy-MIP thin-film microelectrodes was further verified with amperometric i-t measurements, which showed a lower DA levels of detection (10-100 nM) and the low detection limit of 4.5 nM with signal to noise ratio of 3. Good reproducibility with relative standard deviation of 5.45% and long-term stability within two weeks were also observed. The developed OPPy-MIP microelectrode has been successfully applied for acute in vivo DA detection in the striatum of anesthetized rats with 3,4-dihydroxy-l-phenylalanine (l-DOPA) administration, which can be used for future evaluation of novel therapeutic treatments in Parkinsonian rats.
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U2 - 10.1016/j.snb.2011.07.052
DO - 10.1016/j.snb.2011.07.052
M3 - Article
AN - SCOPUS:84864279483
SN - 0925-4005
VL - 171-172
SP - 93
EP - 101
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -