Modification of platinum microelectrode with molecularly imprinted over-oxidized polypyrrole for dopamine measurement in rat striatum

Tien Chun Tsai, Huan Zhang Han, Ching Ching Cheng, Li Chia Chen, Hsien Chang Chang, Jia Jin Jason Chen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A highly sensitive and selective electrochemical microelectrode using the molecularly imprinted (MIP) electropolymer of over-oxidized polypyrrole (OPPy) was proposed for in vivo detection of dopamine (DA) which process is different from current approaches for in vitro DA detection using MIP-based macroelectrode. The proposed MIP electrosynthesized by our present method under a DA/pyrrole-monomer concentration ratio of 1 mM/10 mM yielded the best results for DA adsorption, reaching 2.6-fold that of similar microelectrodes without imprinting. Differential normal pulse voltammetry techniques was used to evaluate the DA-imprinted OPPy microelectrodes for demonstrating high selectivity for in vitro DA detection under conditions with various competitive electroactive molecules. The validity of the modified OPPy-MIP thin-film microelectrodes was further verified with amperometric i-t measurements, which showed a lower DA levels of detection (10-100 nM) and the low detection limit of 4.5 nM with signal to noise ratio of 3. Good reproducibility with relative standard deviation of 5.45% and long-term stability within two weeks were also observed. The developed OPPy-MIP microelectrode has been successfully applied for acute in vivo DA detection in the striatum of anesthetized rats with 3,4-dihydroxy-l-phenylalanine (l-DOPA) administration, which can be used for future evaluation of novel therapeutic treatments in Parkinsonian rats.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume171-172
DOIs
Publication statusPublished - 2012 Aug 1

Fingerprint

dopamine
Microelectrodes
polypyrroles
Polypyrroles
Platinum
rats
Rats
Dopamine
platinum
Pyrroles
phenylalanine
polypyrrole
pyrroles
Voltammetry
Phenylalanine
standard deviation
Signal to noise ratio
signal to noise ratios
monomers
Monomers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

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title = "Modification of platinum microelectrode with molecularly imprinted over-oxidized polypyrrole for dopamine measurement in rat striatum",
abstract = "A highly sensitive and selective electrochemical microelectrode using the molecularly imprinted (MIP) electropolymer of over-oxidized polypyrrole (OPPy) was proposed for in vivo detection of dopamine (DA) which process is different from current approaches for in vitro DA detection using MIP-based macroelectrode. The proposed MIP electrosynthesized by our present method under a DA/pyrrole-monomer concentration ratio of 1 mM/10 mM yielded the best results for DA adsorption, reaching 2.6-fold that of similar microelectrodes without imprinting. Differential normal pulse voltammetry techniques was used to evaluate the DA-imprinted OPPy microelectrodes for demonstrating high selectivity for in vitro DA detection under conditions with various competitive electroactive molecules. The validity of the modified OPPy-MIP thin-film microelectrodes was further verified with amperometric i-t measurements, which showed a lower DA levels of detection (10-100 nM) and the low detection limit of 4.5 nM with signal to noise ratio of 3. Good reproducibility with relative standard deviation of 5.45{\%} and long-term stability within two weeks were also observed. The developed OPPy-MIP microelectrode has been successfully applied for acute in vivo DA detection in the striatum of anesthetized rats with 3,4-dihydroxy-l-phenylalanine (l-DOPA) administration, which can be used for future evaluation of novel therapeutic treatments in Parkinsonian rats.",
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Modification of platinum microelectrode with molecularly imprinted over-oxidized polypyrrole for dopamine measurement in rat striatum. / Tsai, Tien Chun; Han, Huan Zhang; Cheng, Ching Ching; Chen, Li Chia; Chang, Hsien Chang; Chen, Jia Jin Jason.

In: Sensors and Actuators, B: Chemical, Vol. 171-172, 01.08.2012, p. 93-101.

Research output: Contribution to journalArticle

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AU - Tsai, Tien Chun

AU - Han, Huan Zhang

AU - Cheng, Ching Ching

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AU - Chang, Hsien Chang

AU - Chen, Jia Jin Jason

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