Modified Nile red staining method for improved visualization of neutral lipid depositions in stratum corneum

Hamm Ming Sheu, Jui-Chen Tsai, Tzu Kai Lin, Tak-Wah Wong, Yu-Yun Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The neutral lipids existing in the intercellular spaces of the stratum corneum (SC) provide a permeability barrier to prevent water loss. Nile red is the most sensitive lipid stain for tissue sections. However, due to the extremely flattened morphology of corneocytes and the resolution limits of the light microscope, Nile red staining is seldom used as a fluorescent probe for the lipid-rich SC. In the this study, we modified the traditional method for visualization of intracellular lipid by adding 4% potassium hydroxide after Nile red staining. This modified method not only allowed visualization of lipids exsting in the intercellular membrane regions and the lateral junctions of the adjoining corneocytes, but also clearly demonstrated small lipid droplets within pathological corneocytes. These features were not observed with the traditional staining method. Thus, this modified Nile red staining method greatly improved the resolution of the SC lipids under light microscopy and should be useful for studying lipid depositions in both normal and pathological SC.

Original languageEnglish
Pages (from-to)656-660
Number of pages5
JournalJournal of the Formosan Medical Association
Volume102
Issue number9
Publication statusPublished - 2003 Sep

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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