Modulation of energy deficiency in Huntington's disease via activation of the peroxisome proliferator-activated receptor gamma

Ming Chang Chiang, Chiung Mei Chen, Maw Rong Lee, Hsiao Wen Chen, Hui Mei Chen, Yu Shuo Wu, Cheng Han Hung, Jheng Jie Kang, Ching Pang Chang, Chen Chang, Yih Ru Wu, Yau Sheng Tsai, Yijuang Chern

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Huntington's disease (HD) is a neurodegenerative disease caused by the expansion of a CAG trinucleotide repeat in exon 1 of the huntingtin (HTT) gene. Here, we report that the transcript of the peroxisome proliferator-activated receptor-g (PPARγ), a transcription factor that is critical for energy homeostasis, was markedly downregulated in multiple tissues of a mouse model (R6/2) of HD and in lymphocytes of HD patients. Therefore, downregulation of PPARγ seems to be a pathomechanism of HD. Chronic treatment of R6/2 mice with an agonist of PPARγ (thiazolidinedione, TZD) rescued progressive weight loss, motor deterioration, formation of mutant Htt aggregates, jeopardized global ubiquitination profiles, reduced expression of two neuroprotective proteins (brain-derived neurotrophic factor and Bcl-2) and shortened life span exhibited by these mice. By reducing HTT aggregates and, thus, ameliorating the recruitment of PPARγ into HTT aggregates, chronic TZD treatment also elevated the availability of the PPARγ protein and subsequently normalized the expression of two of its downstream genes (the glucose transporter type 4 and PPARγ coactivator-1 alpha genes). The protective effects described above appear to have been exerted, at least partially, via direct activation of PPARγ in the brain, as TZD was detected in the brains of mice treated with TZD and because a PPARγ agonist (rosiglitazone) protected striatal cells from mHTT-evoked energy deficiency and toxicity. We demonstrated that the systematic downregulation of PPARγ seems to play a critical role in the dysregulation of energy homeostasis observed in HD, and that PPARγ is a potential therapeutic target for this disease.

Original languageEnglish
Article numberddq322
Pages (from-to)4043-4058
Number of pages16
JournalHuman Molecular Genetics
Volume19
Issue number20
DOIs
Publication statusPublished - 2010 Jul 28

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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