TY - JOUR
T1 - Molecular characterization of the Pb recombination hotspot in the mouse major histocompatibility complex class II region
AU - Isobe, Taku
AU - Yoshino, Masayasu
AU - Mizuno, Ken Ichi
AU - Fischer Lindahl, Kirsten
AU - Koide, Tsuyoshi
AU - Gaudieri, Silvana
AU - Gojobori, Takashi
AU - Shiroishi, Toshihiko
PY - 2002
Y1 - 2002
N2 - In the mouse major histocompatibility complex (MHC) class II region, meiotic recombination breakpoints are clustered in four specific sites known as hotspots. Here we reveal the primary structure of a hotspot near the Pb gene. A total of 12 crossover points were found to be confined to a 15-kb DNA segment of the Pb pseudogene. Moreover, the crossover points are concentrated in a 341-bp segment, which includes a part of exon 4 and intron 4 of the Pb gene. All four MHC hotspots appear to be located within genes or at the 3′ end of genes, contrasting with characterized hotspots in budding yeast, which are mostly located at the 5′-promoter regions of genes. The Pb hotspot has several consensus motifs, an octamer transcription factor-binding sequence, the B-motif-like transcription factor-binding sequence, and tandem repeats of tetramer sequence - all of which are shared by the other three hotspots. Systematic analysis of the public database demonstrated that the full motif set occurs rarely in the nucleotide sequence of the entire MHC class II region. All results suggest that the motif set has an indispensable role in determining their site specificity.
AB - In the mouse major histocompatibility complex (MHC) class II region, meiotic recombination breakpoints are clustered in four specific sites known as hotspots. Here we reveal the primary structure of a hotspot near the Pb gene. A total of 12 crossover points were found to be confined to a 15-kb DNA segment of the Pb pseudogene. Moreover, the crossover points are concentrated in a 341-bp segment, which includes a part of exon 4 and intron 4 of the Pb gene. All four MHC hotspots appear to be located within genes or at the 3′ end of genes, contrasting with characterized hotspots in budding yeast, which are mostly located at the 5′-promoter regions of genes. The Pb hotspot has several consensus motifs, an octamer transcription factor-binding sequence, the B-motif-like transcription factor-binding sequence, and tandem repeats of tetramer sequence - all of which are shared by the other three hotspots. Systematic analysis of the public database demonstrated that the full motif set occurs rarely in the nucleotide sequence of the entire MHC class II region. All results suggest that the motif set has an indispensable role in determining their site specificity.
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U2 - 10.1006/geno.2002.6817
DO - 10.1006/geno.2002.6817
M3 - Article
C2 - 12160737
AN - SCOPUS:0036989929
SN - 0888-7543
VL - 80
SP - 229
EP - 235
JO - Genomics
JF - Genomics
IS - 2
ER -