Molecular cloning and characterization of orange-spotted grouper (Epinephelus coioides) CXC chemokine ligand 12

Chen Shiou Wu, Ting Yu Wang, Chin Feng Liu, Hao Ping Lin, Young Mao Chen, Tzong-Yueh Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chemokines are a family of soluble peptides that can recruit a wide range of immune cells to sites of infection and disease. The CXCL12 is a chemokine that binds to its cognate receptor CXCR4 and thus involved in multiple physiological and pathophysiological processes. In this study, we cloned and characterized CXCL12 from Epinephelus coioides (osgCXCL12). We found that the open reading frame of osgCXCL12 consists of 98 amino acid residues with the small cytokine C-X-C domain located between residues 29 and 87. Higher expression levels for osgCXCL12 were detected at the kitting stage, compared with the prolarva and larva shape stages. The expression patterns revealed that osgCXCL12 may play a key role in early grouper development. We detected mRNA transcripts for osgCXCL12 in healthy tissues and found the highest osgCXCL12 expression in the head kidney. Furthermore, a time-course analysis revealed significantly increased osgCXCL12 and osgCXCR4 expression levels after the nervous necrosis virus (NNV) challenge. In addition, expression of osgCXCL12 was affected by injection with microbial mimics [LPS and poly(I:C)]. These results suggest that osgCXCL12 is associated with inflammatory and developmental processes in the grouper.

Original languageEnglish
Pages (from-to)996-1005
Number of pages10
JournalFish and Shellfish Immunology
Volume47
Issue number2
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Epinephelus coioides
CXC Chemokines
grouper
Cloning
chemokines
Chemokines
peptide
ligand
molecular cloning
virus
amino acid
CXCR4 Receptors
Ligands
larva
polyinosinic-polycytidylic acid
Poly I-C
Viruses
open reading frames
necrosis
cytokines

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

Wu, Chen Shiou ; Wang, Ting Yu ; Liu, Chin Feng ; Lin, Hao Ping ; Chen, Young Mao ; Chen, Tzong-Yueh. / Molecular cloning and characterization of orange-spotted grouper (Epinephelus coioides) CXC chemokine ligand 12. In: Fish and Shellfish Immunology. 2015 ; Vol. 47, No. 2. pp. 996-1005.
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Molecular cloning and characterization of orange-spotted grouper (Epinephelus coioides) CXC chemokine ligand 12. / Wu, Chen Shiou; Wang, Ting Yu; Liu, Chin Feng; Lin, Hao Ping; Chen, Young Mao; Chen, Tzong-Yueh.

In: Fish and Shellfish Immunology, Vol. 47, No. 2, 01.12.2015, p. 996-1005.

Research output: Contribution to journalArticle

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