TY - JOUR
T1 - Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
AU - Reyes, Alisha Wehdnesday Bernardo
AU - Simborio, Hannah Leah Tadeja
AU - Hop, Huynh Tan
AU - Arayan, Lauren Togonon
AU - Kim, Suk
N1 - Funding Information:
The work was supported the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Korea
Publisher Copyright:
© 2016. The Korean Society of Veterinary Science. All Rights Reserved.
PY - 2016
Y1 - 2016
N2 - The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.
AB - The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.
UR - https://www.scopus.com/pages/publications/84962779534
UR - https://www.scopus.com/pages/publications/84962779534#tab=citedBy
U2 - 10.4142/JVS.2016.17.1.
DO - 10.4142/JVS.2016.17.1.
M3 - Short survey
C2 - 27051349
AN - SCOPUS:84962779534
SN - 1229-845X
VL - 17
SP - 119
EP - 122
JO - Journal of Veterinary Science
JF - Journal of Veterinary Science
IS - 1
ER -