Molecular damage and responses of oral keratinocyte to hydrogen peroxide

Kuan Yu Lin, Ching Hung Chung, Jheng Sian Ciou, Pei Fang Su, Pei Wen Wang, Dar Bin Shieh, Tzu Chueh Wang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Hydrogen peroxide (H2O2)-based tooth bleaching reagents have recently increased in popularity and controversy. H2O2 gel (3%) is used in a Nightguard for vital bleaching; transient tooth sensitivity and oral mucosa irritation have been reported. Genotoxicity and carcinogenicity have also been significant concerns. Methods: We used primary cultured normal human oral keratinocytes (NHOKs) as an in vitro model to investigate the pathological effects to mitochondria functions on human oral keratinocytes exposed to different doses of H2O2 for different durations. Results: An MTT assay showed compromised cell viability at a dose over 5 mM. The treatments induced nuclear DNA damage, measured using a single-cell gel electrophoresis assay. A real-time quantitative polymerase chain reaction showed H2O2 induced significant increase in mitochondrial 4977-bp deletion. Mitochondrial membrane potential and apoptosis assays suggested that oxidative damage defense mechanisms were activated after prolonged exposure to H2O2. Reduced intracellular glutathione was an effective defense against oxidative damage from 5 mM of H2O2. Conclusion: Our study suggests the importance for keratinocyte damage of the dose and the duration of the exposure to H2O2 in at-home-bleaching. A treatment dose ≥100 mM directly causes severe cytotoxicity with as little as 15 min of exposure.

Original languageEnglish
Article number10
JournalBMC Oral Health
Volume19
Issue number1
DOIs
Publication statusPublished - 2019 Jan 11

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Keratinocytes
Hydrogen Peroxide
Dentin Sensitivity
Tooth Bleaching
Comet Assay
Mitochondrial Membrane Potential
Mouth Mucosa
DNA Damage
Glutathione
Real-Time Polymerase Chain Reaction
Cell Survival
Mitochondria
Gels
Apoptosis

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Lin, Kuan Yu ; Chung, Ching Hung ; Ciou, Jheng Sian ; Su, Pei Fang ; Wang, Pei Wen ; Shieh, Dar Bin ; Wang, Tzu Chueh. / Molecular damage and responses of oral keratinocyte to hydrogen peroxide. In: BMC Oral Health. 2019 ; Vol. 19, No. 1.
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Molecular damage and responses of oral keratinocyte to hydrogen peroxide. / Lin, Kuan Yu; Chung, Ching Hung; Ciou, Jheng Sian; Su, Pei Fang; Wang, Pei Wen; Shieh, Dar Bin; Wang, Tzu Chueh.

In: BMC Oral Health, Vol. 19, No. 1, 10, 11.01.2019.

Research output: Contribution to journalArticle

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AU - Lin, Kuan Yu

AU - Chung, Ching Hung

AU - Ciou, Jheng Sian

AU - Su, Pei Fang

AU - Wang, Pei Wen

AU - Shieh, Dar Bin

AU - Wang, Tzu Chueh

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