Molecular diagnosis of primary liver cancer by microsatellite DNA analysis in the serum

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Frequent loss of heterozygosity of microsatellites markers on specific chromosomal region have been reported in various types of primary human cancer. The same loss of heterozygosity has also been identified in the matched plasma/serum DNA. Using 109 microsatellite markers representing 24 chromosomal arms, we have examined the loss of heterozygosity in 21 cases of hepatocellular carcinoma, six of cholangiocarcinoma, and 27 cases of chronic hepatitis or cirrhosis. All cases of the hepatocellular carcinoma showed deletion from two to 10 chromosomal arms, while deletion of chromosomes from two to eight regions was detected in five of six cholangiocarcinoma patients. One or more loss of heterozygosity in the paired serum DNA could be detected in 16 of 25 (76.2%) hepatocellular carcinoma patients. In contrast, no alterations in serum DNA test could be found in cholangiocarcinoma patients. Five of seven (71.4%) hepatocellular carcinoma patients with alpha-fetoprotein levels less than 20 ng ml-1 produced positive serum DNA test. The profiles of 19 microsatellite markers gave a 100% positive predictive value and an 80.8% negative predictive value for hepatocellular carcinoma. In conclusion, we have determined a profile of microsatellite markers appropriate for differential diagnosis of primary liver cancer. The discovery may permit a high-throughput screening of hepatocellular carcinoma at an early stage of disease.

Original languageEnglish
Pages (from-to)1449-1453
Number of pages5
JournalBritish Journal of Cancer
Volume87
Issue number12
DOIs
Publication statusPublished - 2002 Dec 2

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Liver Neoplasms
Microsatellite Repeats
Hepatocellular Carcinoma
Loss of Heterozygosity
Cholangiocarcinoma
DNA
Serum
Chromosome Deletion
alpha-Fetoproteins
Chronic Hepatitis
Differential Diagnosis
Fibrosis
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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title = "Molecular diagnosis of primary liver cancer by microsatellite DNA analysis in the serum",
abstract = "Frequent loss of heterozygosity of microsatellites markers on specific chromosomal region have been reported in various types of primary human cancer. The same loss of heterozygosity has also been identified in the matched plasma/serum DNA. Using 109 microsatellite markers representing 24 chromosomal arms, we have examined the loss of heterozygosity in 21 cases of hepatocellular carcinoma, six of cholangiocarcinoma, and 27 cases of chronic hepatitis or cirrhosis. All cases of the hepatocellular carcinoma showed deletion from two to 10 chromosomal arms, while deletion of chromosomes from two to eight regions was detected in five of six cholangiocarcinoma patients. One or more loss of heterozygosity in the paired serum DNA could be detected in 16 of 25 (76.2{\%}) hepatocellular carcinoma patients. In contrast, no alterations in serum DNA test could be found in cholangiocarcinoma patients. Five of seven (71.4{\%}) hepatocellular carcinoma patients with alpha-fetoprotein levels less than 20 ng ml-1 produced positive serum DNA test. The profiles of 19 microsatellite markers gave a 100{\%} positive predictive value and an 80.8{\%} negative predictive value for hepatocellular carcinoma. In conclusion, we have determined a profile of microsatellite markers appropriate for differential diagnosis of primary liver cancer. The discovery may permit a high-throughput screening of hepatocellular carcinoma at an early stage of disease.",
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Molecular diagnosis of primary liver cancer by microsatellite DNA analysis in the serum. / Chang, Y. C.; Ho, Chung-Liang; Chen, Helen H.W; Chang, Ting-Tsung; Lai, Wu-Wei; Dai, Y. C.; Lee, W. Y.; Chow, Nan-Haw.

In: British Journal of Cancer, Vol. 87, No. 12, 02.12.2002, p. 1449-1453.

Research output: Contribution to journalArticle

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