Molecular diagnostic markers for lung cancer in sputum and plasma

Yi-Ching Wang, Han Shui Hsu, Tsz Pei Chen, Jung Ta Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

46 Citations (Scopus)

Abstract

Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation-specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.

Original languageEnglish
Title of host publicationCirculating Nucleic Acids in Plasma and Serum IV
PublisherBlackwell Publishing Inc.
Pages179-184
Number of pages6
ISBN (Print)157331627X, 9781573316279
DOIs
Publication statusPublished - 2006 Jan 1

Publication series

NameAnnals of the New York Academy of Sciences
Volume1075
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Molecular Pathology
Biomarkers
Sputum
Lung Neoplasms
Microsatellite Repeats
Plasmas
Microsatellite Instability
Methylation
Loss of Heterozygosity
Tumors
Sensitivity and Specificity
Tumor Suppressor Genes
Genetic Markers
Early Detection of Cancer
Cause of Death
Neoplasms
Genes
Tissue
Polymerase Chain Reaction
Lung

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Wang, Y-C., Hsu, H. S., Chen, T. P., & Chen, J. T. (2006). Molecular diagnostic markers for lung cancer in sputum and plasma. In Circulating Nucleic Acids in Plasma and Serum IV (pp. 179-184). (Annals of the New York Academy of Sciences; Vol. 1075). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1368.024
Wang, Yi-Ching ; Hsu, Han Shui ; Chen, Tsz Pei ; Chen, Jung Ta. / Molecular diagnostic markers for lung cancer in sputum and plasma. Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc., 2006. pp. 179-184 (Annals of the New York Academy of Sciences).
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Wang, Y-C, Hsu, HS, Chen, TP & Chen, JT 2006, Molecular diagnostic markers for lung cancer in sputum and plasma. in Circulating Nucleic Acids in Plasma and Serum IV. Annals of the New York Academy of Sciences, vol. 1075, Blackwell Publishing Inc., pp. 179-184. https://doi.org/10.1196/annals.1368.024

Molecular diagnostic markers for lung cancer in sputum and plasma. / Wang, Yi-Ching; Hsu, Han Shui; Chen, Tsz Pei; Chen, Jung Ta.

Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc., 2006. p. 179-184 (Annals of the New York Academy of Sciences; Vol. 1075).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wang Y-C, Hsu HS, Chen TP, Chen JT. Molecular diagnostic markers for lung cancer in sputum and plasma. In Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc. 2006. p. 179-184. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1368.024