Molecular directionality of β-chitin biosynthesis

Junji Sugiyama, Claire Boisset, Masayuki Hashimoto, Takeshi Watanabe

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The molecular packing in β-chitin unit cells was experimentally determined by a combination of unidirectional degradation by Bacillus circulans chitinase A1 and microdiffraction electron crystallography using highly crystalline β-chitin microfibrils from the protective tubes secreted by Lamellibrachia satsuma. The mode of chain packing was found to be identical with that of the previously published crystal model for β-chitin, despite a controversial definition of the unit cell parameters. Here, a 'parallel-down' packing was determined, where the reducing ends of chains point in an opposite direction to the crystallographic c-axis. Microdiffraction analyses of nascent β-chitin microfibrils generated from diatom Thalassiosira sp. showed that the c-axis of the crystal was directed toward the diatoms, and therefore the reducing end of a growing chain pointed away from the locus of biosynthesis. This mechanism agreed well with what we found recently in the cellulose biosynthesis system, and provides strong evidence that the polymerization by the processive glycosyl transferase takes place at the non-reducing end of the growing polysaccharide chains.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalJournal of Molecular Biology
Volume286
Issue number1
DOIs
Publication statusPublished - 1999 Feb 12

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Chitin
Microfibrils
Diatoms
Chitinases
Crystallography
Transferases
Cellulose
Polymerization
Bacillus
Polysaccharides
Electrons

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Sugiyama, Junji ; Boisset, Claire ; Hashimoto, Masayuki ; Watanabe, Takeshi. / Molecular directionality of β-chitin biosynthesis. In: Journal of Molecular Biology. 1999 ; Vol. 286, No. 1. pp. 247-255.
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Molecular directionality of β-chitin biosynthesis. / Sugiyama, Junji; Boisset, Claire; Hashimoto, Masayuki; Watanabe, Takeshi.

In: Journal of Molecular Biology, Vol. 286, No. 1, 12.02.1999, p. 247-255.

Research output: Contribution to journalArticle

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