Molecular epidemiology of plasma oncoproteins in vinyl chloride monomer workers in Taiwan

Jiin Chyuan John Luo, Tsun Jen Cheng, Chung Li Du, Jung-Der Wang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53-Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM-exposed workers. Results: Twenty-five out of 251 (10%) VCM-workers were positive for Asp13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 (15.8%) plasma-positives among the more highly exposed (>480 ppm-month) workers and 10 out of 156 (6.4%) plasma-positives among the lesser exposed (≤480 ppm-month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% CI) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty-three out of 251 (13.2%) VCM-workers were positive for the p53 over-expression (10% with positive p53-Ag and 2.8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp13-p21-ki-ras oncoprotein and p53 over-expression (p53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose-response relationship exists between plasma oncoproteins expression and VCM exposure.

Original languageEnglish
Pages (from-to)94-101
Number of pages8
JournalCancer Detection and Prevention
Volume27
Issue number2
DOIs
Publication statusPublished - 2003 Jan 1

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Vinyl Chloride
Molecular Epidemiology
Oncogene Proteins
Taiwan
Proto-Oncogene Proteins p21(ras)
Tumor Suppressor Protein p53
Drinking
Antibodies
Mutant Proteins
Luminescence
Hepatitis
Anti-Idiotypic Antibodies
Western Blotting
Smoking
Enzyme-Linked Immunosorbent Assay
Odds Ratio

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Luo, Jiin Chyuan John ; Cheng, Tsun Jen ; Du, Chung Li ; Wang, Jung-Der. / Molecular epidemiology of plasma oncoproteins in vinyl chloride monomer workers in Taiwan. In: Cancer Detection and Prevention. 2003 ; Vol. 27, No. 2. pp. 94-101.
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abstract = "Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53-Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM-exposed workers. Results: Twenty-five out of 251 (10{\%}) VCM-workers were positive for Asp13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 (15.8{\%}) plasma-positives among the more highly exposed (>480 ppm-month) workers and 10 out of 156 (6.4{\%}) plasma-positives among the lesser exposed (≤480 ppm-month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95{\%} CI) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty-three out of 251 (13.2{\%}) VCM-workers were positive for the p53 over-expression (10{\%} with positive p53-Ag and 2.8{\%} with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp13-p21-ki-ras oncoprotein and p53 over-expression (p53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose-response relationship exists between plasma oncoproteins expression and VCM exposure.",
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Molecular epidemiology of plasma oncoproteins in vinyl chloride monomer workers in Taiwan. / Luo, Jiin Chyuan John; Cheng, Tsun Jen; Du, Chung Li; Wang, Jung-Der.

In: Cancer Detection and Prevention, Vol. 27, No. 2, 01.01.2003, p. 94-101.

Research output: Contribution to journalArticle

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T1 - Molecular epidemiology of plasma oncoproteins in vinyl chloride monomer workers in Taiwan

AU - Luo, Jiin Chyuan John

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AU - Du, Chung Li

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N2 - Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53-Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM-exposed workers. Results: Twenty-five out of 251 (10%) VCM-workers were positive for Asp13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 (15.8%) plasma-positives among the more highly exposed (>480 ppm-month) workers and 10 out of 156 (6.4%) plasma-positives among the lesser exposed (≤480 ppm-month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% CI) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty-three out of 251 (13.2%) VCM-workers were positive for the p53 over-expression (10% with positive p53-Ag and 2.8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp13-p21-ki-ras oncoprotein and p53 over-expression (p53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose-response relationship exists between plasma oncoproteins expression and VCM exposure.

AB - Aims: To determine the presence of Asp13-p21-ki-ras oncoprotein and p53 oncoprotein in the plasma of vinyl chloride monomer (VCM)-workers in Taiwan. Methods: We used enhanced chemiluminescence (ECL) western blotting to detect Asp13-p21-ki-ras and ELISA to detect mutant p53 protein (p53-Ag) and anti-p53 antibodies (p53-Ab) in the plasma of VCM-exposed workers. Results: Twenty-five out of 251 (10%) VCM-workers were positive for Asp13-p21-ki-ras in plasma, but 0 out of 36 controls were positive. There were 15 out of 95 (15.8%) plasma-positives among the more highly exposed (>480 ppm-month) workers and 10 out of 156 (6.4%) plasma-positives among the lesser exposed (≤480 ppm-month). Compared to the unexposed controls, age and drinking adjusted odds ratios (95% CI) were 1.2 (0.1, 9.8) in the lower exposed workers, and 4.8 (0.8, 28) in the higher exposed workers, and there was a significant linear trend between exposure and plasma positivity (P = 0.001). Thirty-three out of 251 (13.2%) VCM-workers were positive for the p53 over-expression (10% with positive p53-Ag and 2.8% with positive p53-Ab). There was a significant association between cumulative VCM exposure concentration and positive p53 expression (P = 0.032) among VCM-workers after adjusting for age, hepatitis, drinking and smoking status. Conclusions: Asp13-p21-ki-ras oncoprotein and p53 over-expression (p53-Ag or p53-Ab) can be found in the plasma of VCM-workers in Taiwan, and a significant dose-response relationship exists between plasma oncoproteins expression and VCM exposure.

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