Molecular mechanism of the interactions between white spot syndrome virus anti-apoptosis protein AAP-1 (WSSV449) and shrimp effector caspase

Jiann Horng Leu, Li Li Chen, Ying Ru Lin, Guang Hsiung Kou, Chu Fang Lo

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

AAP-1 (WSSV449), an anti-apoptosis protein encoded by white spot syndrome virus (WSSV), blocked apoptosis in insect cells (SF9) induced by Penaeus monodon effector caspase (Pm caspase). Here, to characterize in detail the anti-Pm caspase activity of AAP-1, both proteins were expressed and purified from Escherichia coli and their interactions were assayed in vitro. We found that although AAP-1 could inhibit Pm caspase activity, the inhibition was not as efficient as that of baculovirus anti-apoptosis protein P35. We further confirmed the binding and cleavage of AAP-1 by Pm caspase, and detected three AAP-1 cleavage products. Mutational analysis and protein N-terminal sequencing revealed that whereas both Asp233 and Asp272 residues of AAP-1 are involved in binding and cleavage by Pm caspase, only the Asp272 is involved in Pm caspase inhibition. Asp233, on the other hand, negatively regulates AAP-1's anti-Pm caspase activity. Lastly, AAP-1 homotypically interacts with each other both in vitro and in insect cells.

Original languageEnglish
Pages (from-to)1068-1074
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume34
Issue number10
DOIs
Publication statusPublished - 2010 Oct

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

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