Molecular modeling of the Aβ1-42 peptide from Alzheimer's disease

Michael O. Chaney, Scott D. Webster, Yu Min Kuo, Alex E. Roher

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

The three-dimensional structure of the Alzheimer's disease Aβ1-42 peptide was predicted by sequence homology, threading approaches and by experimental observations. The Aβ molecule displayed a Greek key motif with four antiparallel β-strands. To shield thermodynamically unfavorable domains, two Aβ molecules interact with each other to generate a β-barrel structure with a hydrophilic surface and a hydrophobic core. The N-terminal domains of the dimer form crevices into which the non-polar C-termini are accommodated to yield a globular structure 27 X 32 Å in diameter. Alternatively, the C-terminal domains of two opposing dimers could be extended to form an antiparallel β-sheet. The stacking of these building blocks generates a helical protofilament. To create a thermodynamically more favorable structure, three protofilaments associate into a right-handed triple helix with a hydrophobic β-sheet completely surrounded by the hydrophilic β-barrels made of residues 1-28. Two triple helical strands can further associate into a right-handed amyloid filament. Although our model did not meet all the expected criteria, it nevertheless exhibited a series of naturally disposed structural features, revealed by other biophysical studies utilizing synthetic Aβ peptides. These characteristics are of functional significance in terms of Aβ-topology, fibril formation and cytotoxicity. The model also suggests that Aβ may not exist in a thermodynamically stable conformation, but rather as an ensemble of metastable dimeric structures some of which are capable of generating an extended C-terminal antiparallel β-sheet essential in the promotion of fibrillogenesis.

Original languageEnglish
Pages (from-to)761-767
Number of pages7
JournalProtein Engineering
Volume11
Issue number9
Publication statusPublished - 1998 Sep

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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