Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system

Mei Hwa Lee, Danny O'Hare, Yi Li Chen, Yu Chia Chang, Chien Hsin Yang, Bin Da Liu, Hung Yin Lin

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Melatonin levels may be related to the risks of breast cancer and prostate cancer. The measurement of urinary melatonin is also useful in monitoring serum melatonin levels following oral administration. In this work, melatonin is the target molecule, which is imprinted onto poly(ethylene-co-vinyl alcohol) by evaporation of the solvent on the working electrode of an electrochemical sensing chip. This sensing chip is used directly as a tool for optimizing the imprinting polymer composition, flow rate, and injection volume of the samples. Microfluidic sensing of the target and interference molecules revealed that the lowest detection limit is as low as ~pM, and the electrochemical response is weak even at high interference concentrations. Poly(ethylene-co-vinyl alcohol), containing 44 mol. % ethylene, had an imprinting effectiveness of more than six-fold. In random urine analysis, the microfluidic amperometric measurements of melatonin levels with an additional and recovery of melatonin, the melatonin recovery achieved 94.78± 1.9% for melatonin at a concentration of 1.75-2.11 pg/mL.

Original languageEnglish
Article number054115
Issue number5
Publication statusPublished - 2014 Oct 15

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry


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