Monitoring bioactivity in hydrogen fermentation by an amperometric method with polyviologen modified working electrode

Ming Der Bai, Yung Fu Wang, Chia Jung Hsiao, Shiue Lin Li, Hsien Chang Chang, Sheng Shung Cheng

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

To monitor the bioactivity change of a hydrogen fermentor, this work presents a novel bioelectrochemical method equipped with a polyviologen modified glassy carbon as a working electrode and with ferricyanide as an electron mediator. Experimental results demonstrated that the ferricyanide can transfer electrons from hydrogen producing bacteria cells to electrode without significant inhibition on cell growth. To protect the electrode from fouling by protein and bacteria during bioassay, polyviologen film was applied to modify the working electrode, and stabilized the responding current compared to the use of bare electrode. The amperometric bioassay provided the bioactivity information for indicating the statue of the fermentor and helping draw up the operation strategy when the fermentor was operated under abnormal conditions, including stop-feeding and re-feeding. The results of bioactivity monitoring suggested an interesting phenomenon that the activity of hydrogen producing bacteria transiently increased during the unsteady period and then decreased.

Original languageEnglish
Pages (from-to)1357-1364
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume31
Issue number10
DOIs
Publication statusPublished - 2006 Aug

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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