Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates

Chien-hung Yu, René C.L. Olsthoorn

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Riboswitches are regions within mRNAs that can regulate downstream expression of genes through metabolite-induced alteration of their secondary structures. Due to the significant association of bacterial essential or virulence genes, bacterial riboswitches have become promising targets for development of putative antibacterial drugs. However, most of the screening systems to date are based on in vitro or bacterial systems, lacking the possibility to preobserve the adverse effects to the host's translation machinery. This chapter describes a novel screening method based on monitoring the riboswitch-induced - 1 ribosomal frameshifting (- 1 FS) efficiency in a mammalian cell-free lysate system using preQ1 class-I (preQ1-I) riboswitches as model target.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages385-393
Number of pages9
DOIs
Publication statusPublished - 2015 Jan 1

Publication series

NameMethods in Enzymology
Volume550
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Ribosomal Frameshifting
Riboswitch
Monitoring
Pharmaceutical Preparations
Screening
Genes
Bacterial Genes
Cell-Free System
Metabolites
Machinery
Virulence
Cells
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Yu, C., & Olsthoorn, R. C. L. (2015). Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. In Methods in Enzymology (pp. 385-393). (Methods in Enzymology; Vol. 550). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2014.10.041
Yu, Chien-hung ; Olsthoorn, René C.L. / Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. Methods in Enzymology. Academic Press Inc., 2015. pp. 385-393 (Methods in Enzymology).
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Yu, C & Olsthoorn, RCL 2015, Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. in Methods in Enzymology. Methods in Enzymology, vol. 550, Academic Press Inc., pp. 385-393. https://doi.org/10.1016/bs.mie.2014.10.041

Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. / Yu, Chien-hung; Olsthoorn, René C.L.

Methods in Enzymology. Academic Press Inc., 2015. p. 385-393 (Methods in Enzymology; Vol. 550).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Yu C, Olsthoorn RCL. Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. In Methods in Enzymology. Academic Press Inc. 2015. p. 385-393. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2014.10.041