Abstract
Studies over the last decade using FM dyes to label vesicles at many terminals, including the calyx-type nerve terminal, led to a well accepted "principle" that only a small fraction of vesicles (5-20%) participate in recycling under physiological conditions. This principle imposes a large challenge in maintaining synaptic transmission during repetitive firing, because the small recycling pool may limit the number of available vesicles for release and nerve terminals would have to distinguish the recycling pool from the reserve pool and keep reserve pool vesicles from being used. By recording the presynaptic capacitance changes and the postsynaptic EPSC at rat calyx of Held synapses in the absence or presence of transmitter glutamate in nerve terminals, we developed a new method to count functional recycling vesicles. We found that essentially all vesicles in calyces participated in recycling, challenging the small-recycling-pool principle established by FM dye labeling. Nerve terminals may use all available vesicles to maximize their ability in maintaining synaptic transmission during repetitive firing.
| Original language | English |
|---|---|
| Pages (from-to) | 8820-8826 |
| Number of pages | 7 |
| Journal | Journal of Neuroscience |
| Volume | 33 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2013 May 15 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
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Most vesicles in a central nerve terminal participate in recycling. / Xue, Lei; Sheng, Jiansong; Wu, Xin Sheng; Wu, Wei; Luo, Fujun; Shin, Wonchul; Chinag, HsuehCheng; Wu, Ling Gang.
In: Journal of Neuroscience, Vol. 33, No. 20, 15.05.2013, p. 8820-8826.Research output: Contribution to journal › Article
TY - JOUR
T1 - Most vesicles in a central nerve terminal participate in recycling
AU - Xue, Lei
AU - Sheng, Jiansong
AU - Wu, Xin Sheng
AU - Wu, Wei
AU - Luo, Fujun
AU - Shin, Wonchul
AU - Chinag, HsuehCheng
AU - Wu, Ling Gang
PY - 2013/5/15
Y1 - 2013/5/15
N2 - Studies over the last decade using FM dyes to label vesicles at many terminals, including the calyx-type nerve terminal, led to a well accepted "principle" that only a small fraction of vesicles (5-20%) participate in recycling under physiological conditions. This principle imposes a large challenge in maintaining synaptic transmission during repetitive firing, because the small recycling pool may limit the number of available vesicles for release and nerve terminals would have to distinguish the recycling pool from the reserve pool and keep reserve pool vesicles from being used. By recording the presynaptic capacitance changes and the postsynaptic EPSC at rat calyx of Held synapses in the absence or presence of transmitter glutamate in nerve terminals, we developed a new method to count functional recycling vesicles. We found that essentially all vesicles in calyces participated in recycling, challenging the small-recycling-pool principle established by FM dye labeling. Nerve terminals may use all available vesicles to maximize their ability in maintaining synaptic transmission during repetitive firing.
AB - Studies over the last decade using FM dyes to label vesicles at many terminals, including the calyx-type nerve terminal, led to a well accepted "principle" that only a small fraction of vesicles (5-20%) participate in recycling under physiological conditions. This principle imposes a large challenge in maintaining synaptic transmission during repetitive firing, because the small recycling pool may limit the number of available vesicles for release and nerve terminals would have to distinguish the recycling pool from the reserve pool and keep reserve pool vesicles from being used. By recording the presynaptic capacitance changes and the postsynaptic EPSC at rat calyx of Held synapses in the absence or presence of transmitter glutamate in nerve terminals, we developed a new method to count functional recycling vesicles. We found that essentially all vesicles in calyces participated in recycling, challenging the small-recycling-pool principle established by FM dye labeling. Nerve terminals may use all available vesicles to maximize their ability in maintaining synaptic transmission during repetitive firing.
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U2 - 10.1523/JNEUROSCI.4029-12.2013
DO - 10.1523/JNEUROSCI.4029-12.2013
M3 - Article
VL - 33
SP - 8820
EP - 8826
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 20
ER -