Most vesicles in a central nerve terminal participate in recycling

Lei Xue, Jiansong Sheng, Xin Sheng Wu, Wei Wu, Fujun Luo, Wonchul Shin, HsuehCheng Chinag, Ling Gang Wu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Studies over the last decade using FM dyes to label vesicles at many terminals, including the calyx-type nerve terminal, led to a well accepted "principle" that only a small fraction of vesicles (5-20%) participate in recycling under physiological conditions. This principle imposes a large challenge in maintaining synaptic transmission during repetitive firing, because the small recycling pool may limit the number of available vesicles for release and nerve terminals would have to distinguish the recycling pool from the reserve pool and keep reserve pool vesicles from being used. By recording the presynaptic capacitance changes and the postsynaptic EPSC at rat calyx of Held synapses in the absence or presence of transmitter glutamate in nerve terminals, we developed a new method to count functional recycling vesicles. We found that essentially all vesicles in calyces participated in recycling, challenging the small-recycling-pool principle established by FM dye labeling. Nerve terminals may use all available vesicles to maximize their ability in maintaining synaptic transmission during repetitive firing.

Original languageEnglish
Pages (from-to)8820-8826
Number of pages7
JournalJournal of Neuroscience
Volume33
Issue number20
DOIs
Publication statusPublished - 2013 May 15

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Recycling
Synaptic Transmission
Coloring Agents
Synapses
Glutamic Acid

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Xue, L., Sheng, J., Wu, X. S., Wu, W., Luo, F., Shin, W., ... Wu, L. G. (2013). Most vesicles in a central nerve terminal participate in recycling. Journal of Neuroscience, 33(20), 8820-8826. https://doi.org/10.1523/JNEUROSCI.4029-12.2013
Xue, Lei ; Sheng, Jiansong ; Wu, Xin Sheng ; Wu, Wei ; Luo, Fujun ; Shin, Wonchul ; Chinag, HsuehCheng ; Wu, Ling Gang. / Most vesicles in a central nerve terminal participate in recycling. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 20. pp. 8820-8826.
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Xue, L, Sheng, J, Wu, XS, Wu, W, Luo, F, Shin, W, Chinag, H & Wu, LG 2013, 'Most vesicles in a central nerve terminal participate in recycling', Journal of Neuroscience, vol. 33, no. 20, pp. 8820-8826. https://doi.org/10.1523/JNEUROSCI.4029-12.2013

Most vesicles in a central nerve terminal participate in recycling. / Xue, Lei; Sheng, Jiansong; Wu, Xin Sheng; Wu, Wei; Luo, Fujun; Shin, Wonchul; Chinag, HsuehCheng; Wu, Ling Gang.

In: Journal of Neuroscience, Vol. 33, No. 20, 15.05.2013, p. 8820-8826.

Research output: Contribution to journalArticle

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