MSRB7 reverses oxidation of GSTF2/3 to confer tolerance of Arabidopsis thaliana to oxidative stress

Shu Hong Lee, Chia Wen Li, Kah Wee Koh, Hsin Yu Chuang, Yet Ran Chen, Choun Sea Lin, Ming Tsair Chan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Methionine sulfoxide reductases (MSRs) catalyse the reduction of oxidized methionine residues, thereby protecting proteins against oxidative stress. Accordingly, MSRs have been associated with stress responses, disease, and senescence in a taxonomically diverse array of organisms. However, the cytosolic substrates of MSRs in plants remain largely unknown. Here, we used a proteomic analysis strategy to identify MSRB7 substrates. We showed that two glutathione transferases (GSTs), GSTF2 and GSTF3, had fewer oxidized methionine (MetO) residues in MSRB7- overexpressing Arabidopsis thaliana plants than in wild-type plants. Conversely, GSTF2 and GSTF3 were highly oxidized and unstable in MSRB7-knockdown plants. MSRB7 was able to restore the MetO-GSTF2M100/104 and MetOGSTF3M100 residues produced during oxidative stress. Furthermore, both GSTs were specifically induced by the oxidative stress inducer, methyl viologen. Our results indicate that specific GSTs are substrates of MSRs, which together provide a major line of defence against oxidative stress in A. thaliana.

Original languageEnglish
Pages (from-to)5049-5062
Number of pages14
JournalJournal of experimental botany
Volume65
Issue number17
DOIs
Publication statusPublished - 2014 Sep

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

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