Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression

Yuri Tanaka; Yoshimi Oshima; Tomomichi Yamamura; Masao Sugiyama; Nobutaka Mitsuda; Norihiro Ohtsubo; Masaru Ohme-Takagi; Teruhiko Terakawa

doi:10.1038/srep02641

Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression

Yuri Tanaka, Yoshimi Oshima, Tomomichi Yamamura, Masao Sugiyama, Nobutaka Mitsuda, Norihiro Ohtsubo, Masaru Ohme-Takagi, Teruhiko Terakawa

NCKU-AS Graduate-Program in Translational Agriculture Sciences

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX andCpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.

Original language	English
Article number	2641
Journal	Scientific reports
Volume	3
DOIs	https://doi.org/10.1038/srep02641
Publication status	Published - 2013 Sept 12

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title = "Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression",

abstract = "Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX andCpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.",

author = "Yuri Tanaka and Yoshimi Oshima and Tomomichi Yamamura and Masao Sugiyama and Nobutaka Mitsuda and Norihiro Ohtsubo and Masaru Ohme-Takagi and Teruhiko Terakawa",

note = "Funding Information: This work was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry from the Bio-oriented Technology Research Advancement Institution and also supported by the Research and Development Project for Application in Promoting New Policy of Agriculture, Forestry and Fisheries.",

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doi = "10.1038/srep02641",

language = "English",

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AU - Tanaka, Yuri

AU - Oshima, Yoshimi

AU - Yamamura, Tomomichi

AU - Sugiyama, Masao

AU - Mitsuda, Nobutaka

AU - Ohtsubo, Norihiro

AU - Ohme-Takagi, Masaru

AU - Terakawa, Teruhiko

N1 - Funding Information: This work was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry from the Bio-oriented Technology Research Advancement Institution and also supported by the Research and Development Project for Application in Promoting New Policy of Agriculture, Forestry and Fisheries.

PY - 2013/9/12

Y1 - 2013/9/12

N2 - Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX andCpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.

AB - Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) caused stamens to convert into petals, resulting in a flower with 10 petals. By contrast, CpAG2-SRDX only caused incomplete formation of stamens and carpels. Expression in Arabidopsis thaliana showed similar effects on flower organ specification. Simultaneous expression of CpAG1-SRDX andCpAG2-SRDX in cyclamen induced rose-like, multi-petal flowers, a potentially valuable trait in commercial ornamental varieties. Expression of CpAG2-SRDX in a cyclamen mutant lacking expression of CpAG1 more effectively produced multi-petal flowers. Here, we controlled the number of petals in cyclamen by simple genetic engineering with a chimeric repressor. This strategy may be applicable useful for other ornamental plants with two distinct AG orthologues.

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Multi-petal cyclamen flowers produced by AGAMOUS chimeric repressor expression

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