TY - JOUR
T1 - Multifunctional liposomal drug delivery with dual probes of magnetic resonance and fluorescence imaging
AU - Huang, Chih Ling
AU - Hsieh, Wan Ju
AU - Lin, Che Wei
AU - Yang, Hung Wei
AU - Wang, Chih Kuang
N1 - Funding Information:
The authors gratefully acknowledge the financial support provided to this study by the Ministry of Science and Technology (MOST) in Taiwan under Grant No. MOST- 105–2218-E-037-002. This study is also supported partially by Aim for the Top Universities Grant of Kaohsiung Medical University (KMU-TP105B03) and Kaohsiung Medical University Research Foundation (KMU-Q106007).
Funding Information:
The authors gratefully acknowledge the financial support provided to this study by the Ministry of Science and Technology (MOST) in Taiwan under Grant No. MOST- 105–2218-E-037-002 . This study is also supported partially by Aim for the Top Universities Grant of Kaohsiung Medical University ( KMU-TP105B03 ) and Kaohsiung Medical University Research Foundation ( KMU-Q106007 ).
Publisher Copyright:
© 2018 Elsevier Ltd and Techna Group S.r.l.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Many liposomal drug carriers have shown great promise in the clinic. To ensure the efficient preclinical development of drug-loaded liposomes, the drug retention and circulation properties of these systems should be characterized. Iron oxide (Fe3O4) magnetic nanoparticles (MNPs) are used as T2 contrast agents in magnetic resonance imaging (MRI). Gold nanoclusters (GNCs) contain tens of atoms with subnanometer dimensions; they have very low cytotoxicity and possess superb red emitting fluorescent properties, which prevents in vivo background autofluorescence. The aim of this study was to develop dual imaging, nanocomposite, multifunctional liposome drug carriers (Fe3O4-GNCs) comprising MNPs of iron oxide and GNCs. First, MNPs of iron oxide were synthesized by co-precipitation. The MNP surfaces were modified with amine groups using 3-aminopropyltriethoxysilane (APTES). Second, GNCs were synthesized by reducing HAuCl4·3H2O with NaBH4 in the presence of lipoic acid (as a stabilizer and nanosynthetic template). The GNCs were grown by adsorption onto particles to control the size and stability of the resultant colloids. Subsequently, dual Fe3O4-GNCs imaging probes were fabricated by conjugating the iron oxide MNPs with the GNCs via amide bonds. Finally, liposome nanocarriers were used to enclose the Fe3O4-GNCs in an inner phase (liposome@Fe3O4-GNCs) by reverse phase evaporation. These nanocarriers were characterized by dynamic light scattering (DLS), fluorescence spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectrophotometry, superconducting quantum interference device (SQUID), nuclear magnetic resonance (NMR) imaging and in vivo imaging systems (IVIS). These multifunctional liposomal drug delivery systems with dual probes are expected to prove useful in preclinical trials for cancer diagnosis and therapy.
AB - Many liposomal drug carriers have shown great promise in the clinic. To ensure the efficient preclinical development of drug-loaded liposomes, the drug retention and circulation properties of these systems should be characterized. Iron oxide (Fe3O4) magnetic nanoparticles (MNPs) are used as T2 contrast agents in magnetic resonance imaging (MRI). Gold nanoclusters (GNCs) contain tens of atoms with subnanometer dimensions; they have very low cytotoxicity and possess superb red emitting fluorescent properties, which prevents in vivo background autofluorescence. The aim of this study was to develop dual imaging, nanocomposite, multifunctional liposome drug carriers (Fe3O4-GNCs) comprising MNPs of iron oxide and GNCs. First, MNPs of iron oxide were synthesized by co-precipitation. The MNP surfaces were modified with amine groups using 3-aminopropyltriethoxysilane (APTES). Second, GNCs were synthesized by reducing HAuCl4·3H2O with NaBH4 in the presence of lipoic acid (as a stabilizer and nanosynthetic template). The GNCs were grown by adsorption onto particles to control the size and stability of the resultant colloids. Subsequently, dual Fe3O4-GNCs imaging probes were fabricated by conjugating the iron oxide MNPs with the GNCs via amide bonds. Finally, liposome nanocarriers were used to enclose the Fe3O4-GNCs in an inner phase (liposome@Fe3O4-GNCs) by reverse phase evaporation. These nanocarriers were characterized by dynamic light scattering (DLS), fluorescence spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectrophotometry, superconducting quantum interference device (SQUID), nuclear magnetic resonance (NMR) imaging and in vivo imaging systems (IVIS). These multifunctional liposomal drug delivery systems with dual probes are expected to prove useful in preclinical trials for cancer diagnosis and therapy.
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U2 - 10.1016/j.ceramint.2018.04.034
DO - 10.1016/j.ceramint.2018.04.034
M3 - Article
AN - SCOPUS:85045316566
SN - 0272-8842
VL - 44
SP - 12442
EP - 12450
JO - Ceramics International
JF - Ceramics International
IS - 11
ER -
