Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

Hui Ju Huang; Sen Lin Tang; Yuan Chih Chang; Hao Ching Wang; Tze Hann Ng; Rees F. Garmann; Yu Wen Chen; Jiun Yan Huang; Ramya Kumar; Sheng Hsiung Chang; Shang Rung Wu; Chih Yu Chao; Kyoko Matoba; Iwasaki Kenji; William M. Gelbart; Tzu Ping Ko; Hwei Jiung Wang; Chu Fang Lo; Li Li Chen; Han Ching Wang

doi:10.3390/ijms24087525

Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

Hui Ju Huang, Sen Lin Tang, Yuan Chih Chang, Hao Ching Wang, Tze Hann Ng, Rees F. Garmann, Yu Wen Chen, Jiun Yan Huang, Ramya Kumar, Sheng Hsiung Chang, Shang Rung Wu, Chih Yu Chao, Kyoko Matoba, Iwasaki Kenji, William M. Gelbart, Tzu Ping Ko, Hwei Jiung Wang, Chu Fang Lo, Li Li Chen, Han Ching Wang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.

Original language	English
Article number	7525
Journal	International journal of molecular sciences
Volume	24
Issue number	8
DOIs	https://doi.org/10.3390/ijms24087525
Publication status	Published - 2023 Apr

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms24087525

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Huang, H. J., Tang, S. L., Chang, Y. C., Wang, H. C., Ng, T. H., Garmann, R. F., Chen, Y. W., Huang, J. Y., Kumar, R., Chang, S. H., Wu, S. R., Chao, C. Y., Matoba, K., Kenji, I., Gelbart, W. M., Ko, T. P., Wang, H. J., Lo, C. F., Chen, L. L., & Wang, H. C. (2023). Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway. International journal of molecular sciences, 24(8), Article 7525. https://doi.org/10.3390/ijms24087525

@article{9772c35f6a63408abcbc1e0fc4064afb,

title = "Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway",

abstract = "White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.",

author = "Huang, {Hui Ju} and Tang, {Sen Lin} and Chang, {Yuan Chih} and Wang, {Hao Ching} and Ng, {Tze Hann} and Garmann, {Rees F.} and Chen, {Yu Wen} and Huang, {Jiun Yan} and Ramya Kumar and Chang, {Sheng Hsiung} and Wu, {Shang Rung} and Chao, {Chih Yu} and Kyoko Matoba and Iwasaki Kenji and Gelbart, {William M.} and Ko, {Tzu Ping} and Wang, {Hwei Jiung} and Lo, {Chu Fang} and Chen, {Li Li} and Wang, {Han Ching}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = apr,

doi = "10.3390/ijms24087525",

language = "English",

volume = "24",

journal = "International journal of molecular sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "8",

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Huang, HJ, Tang, SL, Chang, YC, Wang, HC, Ng, TH, Garmann, RF, Chen, YW, Huang, JY, Kumar, R, Chang, SH, Wu, SR, Chao, CY, Matoba, K, Kenji, I, Gelbart, WM, Ko, TP, Wang, HJ, Lo, CF, Chen, LL & Wang, HC 2023, 'Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway', International journal of molecular sciences, vol. 24, no. 8, 7525. https://doi.org/10.3390/ijms24087525

TY - JOUR

T1 - Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

AU - Huang, Hui Ju

AU - Tang, Sen Lin

AU - Chang, Yuan Chih

AU - Wang, Hao Ching

AU - Ng, Tze Hann

AU - Garmann, Rees F.

AU - Chen, Yu Wen

AU - Huang, Jiun Yan

AU - Kumar, Ramya

AU - Chang, Sheng Hsiung

AU - Wu, Shang Rung

AU - Chao, Chih Yu

AU - Matoba, Kyoko

AU - Kenji, Iwasaki

AU - Gelbart, William M.

AU - Ko, Tzu Ping

AU - Wang, Hwei Jiung

AU - Lo, Chu Fang

AU - Chen, Li Li

AU - Wang, Han Ching

PY - 2023/4

Y1 - 2023/4

N2 - White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.

AB - White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.

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U2 - 10.3390/ijms24087525

DO - 10.3390/ijms24087525

M3 - Article

AN - SCOPUS:85158012965

SN - 1661-6596

VL - 24

JO - International journal of molecular sciences

JF - International journal of molecular sciences

IS - 8

M1 - 7525

ER -

Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

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