Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

Hui Ju Huang, Sen Lin Tang, Yuan Chih Chang, Hao Ching Wang, Tze Hann Ng, Rees F. Garmann, Yu Wen Chen, Jiun Yan Huang, Ramya Kumar, Sheng Hsiung Chang, Shang Rung Wu, Chih Yu Chao, Kyoko Matoba, Iwasaki Kenji, William M. Gelbart, Tzu Ping Ko, Hwei Jiung Wang, Chu Fang Lo, Li Li Chen, Han Ching Wang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.

Original languageEnglish
Article number7525
JournalInternational journal of molecular sciences
Volume24
Issue number8
DOIs
Publication statusPublished - 2023 Apr

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway'. Together they form a unique fingerprint.

Cite this