Multiple vesiculoviral matrix proteins inhibit both nuclear export and import

Jeannine M. Petersen, Lu-Shiun Her, James E. Dahlberg

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The matrix (M) protein of vesicular stomatitis virus inhibits both nuclear import and export. Here, we demonstrate that this inhibitory property is conserved between the M proteins from two other vesiculoviruses, chandipura virus and spring viremia carp virus. All three M proteins completely block nuclear transport of spliced mRNA, small nuclear RNAs, and small nuclear ribonucleoproteins and slow the nuclear transport of many other cargoes. In all cases where transport was merely slowed by the M proteins, the chandipura virus M protein had the strongest inhibitory activity. When expressed in transfected HeLa cells, active M proteins displayed prominent association with the nuclear rim. Moreover, mutation of a conserved methionine abolished both the inhibitory activity and efficient targeting of the M proteins to the nuclear rim. We propose that all of the vesiculoviral M proteins associate with the same nuclear target, which is likely to be a component of the nuclear pore complex.

Original languageEnglish
Pages (from-to)8590-8595
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number15
DOIs
Publication statusPublished - 2001 Jul 17

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Cell Nucleus Active Transport
Vesiculovirus
Proteins
Small Nuclear Ribonucleoproteins
Small Nuclear RNA
Nuclear Pore
Carps
Viremia
Protein Transport
Nuclear Proteins
HeLa Cells
Methionine
Viruses
Messenger RNA
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics
  • General

Cite this

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abstract = "The matrix (M) protein of vesicular stomatitis virus inhibits both nuclear import and export. Here, we demonstrate that this inhibitory property is conserved between the M proteins from two other vesiculoviruses, chandipura virus and spring viremia carp virus. All three M proteins completely block nuclear transport of spliced mRNA, small nuclear RNAs, and small nuclear ribonucleoproteins and slow the nuclear transport of many other cargoes. In all cases where transport was merely slowed by the M proteins, the chandipura virus M protein had the strongest inhibitory activity. When expressed in transfected HeLa cells, active M proteins displayed prominent association with the nuclear rim. Moreover, mutation of a conserved methionine abolished both the inhibitory activity and efficient targeting of the M proteins to the nuclear rim. We propose that all of the vesiculoviral M proteins associate with the same nuclear target, which is likely to be a component of the nuclear pore complex.",
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Multiple vesiculoviral matrix proteins inhibit both nuclear export and import. / Petersen, Jeannine M.; Her, Lu-Shiun; Dahlberg, James E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 15, 17.07.2001, p. 8590-8595.

Research output: Contribution to journalArticle

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