Multiplexed Graphene Quantum Dots with Excitation-Wavelength-Independent Photoluminescence, as Two-Photon Probes, and in Ultraviolet-Near Infrared Bioimaging

Wen Shuo Kuo, Xing Can Shen, Chia Yuan Chang, Hui Fang Kao, Sheng Han Lin, Jiu Yao Wang, Ping Ching Wu

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In this study, sorted nitrogen-doped graphene quantum dots were prepared and subsequently conjugated with polymers. The synthesized materials exhibited excitation-wavelength-independent photoluminescence emissions ranging from ultraviolet to near-infrared and were 0.9-8.4 nm in size. The materials also exhibited high-photoluminescence quantum yields and excellent two-photon properties. Therefore, in two-photon bioimaging the materials with different emission spectra can be effective two-photon contrast agents. Specific antibodies were used to label organelles in cancer cells and identify nuclear antigens, thereby enabling the simultaneous detection of four targets in cells at a single two-photon excitation wavelength. The sorted nitrogen-doped graphene quantum dot materials were determined to be considerably more advantageous than organic dyes in identifying multiplexed targets, and they can be effective probes in cellular imaging.

Original languageEnglish
Pages (from-to)11502-11509
Number of pages8
JournalACS nano
Volume14
Issue number9
DOIs
Publication statusPublished - 2020 Sep 22

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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