Multiresolution image analysis for automatic quantification of collagen gel contraction

Hsin Chen Chen, Tai-Hua Yang, Andrew R. Thoreson, Chunfeng Zhao, Peter C. Amadio, Fong-chin Su, Wenyan Jia, Yung-Nien Sun, Kai Nan An, Mingui Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Quantifying collagen gel contraction is important in tissue engineering and biological research because it provides spatial-temporal assessments of cell behaviors and tissue material properties. However, these assessments currently rely on manual processing, which is time-consuming and subjective to personal opinions. We present a multiresolution image analysis system for automatic quantification of gel contraction. This system includes a color conversion process to normalize and enhance the contrast between gel and background. Then, a deformable circular model is activated automatically to capture details of gel boundaries. These steps are coordinated by a multiresolution strategy. The target measurements are obtained after gel segmentation. Our experiments using 30 images demonstrated a high consistency between the proposed and manual segmentation methods. The system can process large-size images (4000x3000) at a rate of approximately one second per image. It thus serves as a useful tool for analyzing large biological and biomaterial imaging datasets efficiently and objectively.

Original languageEnglish
Title of host publicationProceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013
Pages64-65
Number of pages2
DOIs
Publication statusPublished - 2013 Nov 8
Event39th Annual Northeast Bioengineering Conference, NEBEC 2013 - Syracuse, NY, United States
Duration: 2013 Apr 52013 Apr 7

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other39th Annual Northeast Bioengineering Conference, NEBEC 2013
CountryUnited States
CitySyracuse, NY
Period13-04-0513-04-07

Fingerprint

Collagen
Image analysis
Gels
Biocompatible Materials
Tissue engineering
Biomaterials
Materials properties
Tissue
Color
Imaging techniques
Processing
Experiments

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Chen, H. C., Yang, T-H., Thoreson, A. R., Zhao, C., Amadio, P. C., Su, F., ... Sun, M. (2013). Multiresolution image analysis for automatic quantification of collagen gel contraction. In Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013 (pp. 64-65). [6574359] (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC). https://doi.org/10.1109/NEBEC.2013.115
Chen, Hsin Chen ; Yang, Tai-Hua ; Thoreson, Andrew R. ; Zhao, Chunfeng ; Amadio, Peter C. ; Su, Fong-chin ; Jia, Wenyan ; Sun, Yung-Nien ; An, Kai Nan ; Sun, Mingui. / Multiresolution image analysis for automatic quantification of collagen gel contraction. Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013. 2013. pp. 64-65 (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC).
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Chen, HC, Yang, T-H, Thoreson, AR, Zhao, C, Amadio, PC, Su, F, Jia, W, Sun, Y-N, An, KN & Sun, M 2013, Multiresolution image analysis for automatic quantification of collagen gel contraction. in Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013., 6574359, Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC, pp. 64-65, 39th Annual Northeast Bioengineering Conference, NEBEC 2013, Syracuse, NY, United States, 13-04-05. https://doi.org/10.1109/NEBEC.2013.115

Multiresolution image analysis for automatic quantification of collagen gel contraction. / Chen, Hsin Chen; Yang, Tai-Hua; Thoreson, Andrew R.; Zhao, Chunfeng; Amadio, Peter C.; Su, Fong-chin; Jia, Wenyan; Sun, Yung-Nien; An, Kai Nan; Sun, Mingui.

Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013. 2013. p. 64-65 6574359 (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen HC, Yang T-H, Thoreson AR, Zhao C, Amadio PC, Su F et al. Multiresolution image analysis for automatic quantification of collagen gel contraction. In Proceedings - 39th Annual Northeast Bioengineering Conference, NEBEC 2013. 2013. p. 64-65. 6574359. (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC). https://doi.org/10.1109/NEBEC.2013.115