Muscarinic depression of excitatory synaptic transmission mediated by the presynaptic M3 receptors in the rat neostriatum

Kuei-Sen Hsu, Chiung Chun Huang, Po-Wu Gean

Research output: Contribution to journalArticle

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Abstract

The effect of carbachol on the excitatory synaptic transmission was studied in rat neostriatal neurons using intracellular and whole-cell voltage clamp-recording methods. Depolarizing excitatory postsynaptic potentials (EPSPs) were evoked by cortical stimulation. Superfusion of carbachol (0.01-3 μM) reversibly decreases the EPSP amplitude in a concentration-dependent manner and with an estimated IC50 of 0.3 μM. While, neither the N-methyl-d-aspartate (NMDA, 100 μM)- nor (±)-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA, 100 μM)-induced response was affected by carbachol (0.1 μM). In addition, the inhibitory effect induced by carbachol at a low concentration of 0.1 μM. was attenuated by 4-diphenylacetoxy-N,N-methyl-piperidine (4-DAMP), a selective M3 muscarinic receptor antagonist. However, other muscarinic subtype (M1 or M2) antagonists could also block the inhibitory effect by carbachol 0.1 μM. The rank order of antagonist potency was: 4-DAMP (M3 antagonist) > methoctramine (M2 antagonist) > pirenzepine (M1 antagonist). Based on these findings, we conclude that carbachol at a low concentration (≤0.1 μM) reduced the excitatory response of neostriatal neurons following cortical stimulation via presynaptic M3 muscarinic receptors located on the terminals of corticostriatal neurons.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalNeuroscience Letters
Volume197
Issue number2
DOIs
Publication statusPublished - 1995 Sep 8

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Presynaptic Receptors
Neostriatum
Carbachol
Synaptic Transmission
Cholinergic Agents
Excitatory Postsynaptic Potentials
Neurons
Muscarinic M3 Receptors
Pirenzepine
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Muscarinic Antagonists
N-Methylaspartate
Aspartic Acid
Inhibitory Concentration 50

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Muscarinic depression of excitatory synaptic transmission mediated by the presynaptic M3 receptors in the rat neostriatum",
abstract = "The effect of carbachol on the excitatory synaptic transmission was studied in rat neostriatal neurons using intracellular and whole-cell voltage clamp-recording methods. Depolarizing excitatory postsynaptic potentials (EPSPs) were evoked by cortical stimulation. Superfusion of carbachol (0.01-3 μM) reversibly decreases the EPSP amplitude in a concentration-dependent manner and with an estimated IC50 of 0.3 μM. While, neither the N-methyl-d-aspartate (NMDA, 100 μM)- nor (±)-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA, 100 μM)-induced response was affected by carbachol (0.1 μM). In addition, the inhibitory effect induced by carbachol at a low concentration of 0.1 μM. was attenuated by 4-diphenylacetoxy-N,N-methyl-piperidine (4-DAMP), a selective M3 muscarinic receptor antagonist. However, other muscarinic subtype (M1 or M2) antagonists could also block the inhibitory effect by carbachol 0.1 μM. The rank order of antagonist potency was: 4-DAMP (M3 antagonist) > methoctramine (M2 antagonist) > pirenzepine (M1 antagonist). Based on these findings, we conclude that carbachol at a low concentration (≤0.1 μM) reduced the excitatory response of neostriatal neurons following cortical stimulation via presynaptic M3 muscarinic receptors located on the terminals of corticostriatal neurons.",
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Muscarinic depression of excitatory synaptic transmission mediated by the presynaptic M3 receptors in the rat neostriatum. / Hsu, Kuei-Sen; Huang, Chiung Chun; Gean, Po-Wu.

In: Neuroscience Letters, Vol. 197, No. 2, 08.09.1995, p. 141-144.

Research output: Contribution to journalArticle

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