Mushroom body glycolysis is required for olfactory memory in Drosophila

Chia Lin Wu, Ching Ching Chang, Jie Kai Wu, Meng Hsuan Chiang, Chu Huai Yang, HsuehCheng Chinag

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glucose catabolism, also known as glycolysis, is important for energy generation and involves a sequence of enzymatic reactions that convert a glucose molecule into two pyruvate molecules. The glycolysis process generates adenosine triphosphate as a byproduct. In this study, we investigated whether glycolysis plays a role in maintaining neuronal functions in the Drosophila mushroom bodies (MBs), which are generally accepted to be an olfactory learning and memory center. Our data showed that individual knockdown of glycolytic enzymes in the MBs, including hexokinase (HexA), phosphofructokinase (Pfk), or pyruvate kinase (PyK), disrupts olfactory memory. Whole-mount brain immunostaining indicated that pyruvate kinase is strongly expressed in the MB αβ, α′β′, and γ neuron subsets. We conclude that HexA, Pfk, and PyK are required in each MB neuron subset for olfactory memory formation. Our data therefore indicates that glucose catabolism in the MBs is important for olfactory memory formation in Drosophila.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalNeurobiology of Learning and Memory
Volume150
DOIs
Publication statusPublished - 2018 Apr 1

Fingerprint

Mushroom Bodies
Glycolysis
Drosophila
Pyruvate Kinase
Phosphofructokinases
Hexokinase
Glucose
Neurons
Pyruvic Acid
Adenosine Triphosphate
Learning
Brain
Enzymes

All Science Journal Classification (ASJC) codes

  • Experimental and Cognitive Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience

Cite this

Wu, Chia Lin ; Chang, Ching Ching ; Wu, Jie Kai ; Chiang, Meng Hsuan ; Yang, Chu Huai ; Chinag, HsuehCheng. / Mushroom body glycolysis is required for olfactory memory in Drosophila. In: Neurobiology of Learning and Memory. 2018 ; Vol. 150. pp. 13-19.
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Mushroom body glycolysis is required for olfactory memory in Drosophila. / Wu, Chia Lin; Chang, Ching Ching; Wu, Jie Kai; Chiang, Meng Hsuan; Yang, Chu Huai; Chinag, HsuehCheng.

In: Neurobiology of Learning and Memory, Vol. 150, 01.04.2018, p. 13-19.

Research output: Contribution to journalArticle

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