Myostatin gene organization and nodavirus-influenced expression in orange-spotted grouper (Epinephelus coioides)

Y. M. Chen; C. Y. Wei; C. H. Chien; H. W. Chang; S. I. Huang; H. L. Yang; T. Y. Chen

doi:10.1016/j.cbd.2007.04.003

Myostatin gene organization and nodavirus-influenced expression in orange-spotted grouper (Epinephelus coioides)

Y. M. Chen, C. Y. Wei, C. H. Chien, H. W. Chang, S. I. Huang, H. L. Yang, T. Y. Chen

Department of Biotechnology and Bioindustry Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The relationship(s) between nodavirus infection and myostatin expression in the skeletal muscle tissue of grouper is unclear. To investigate, the grouper (Epinephelus coioides) myostatin gene was cloned and cDNA was utilized to examine the expression of the gene in skeletal muscle and serum of healthy (uninfected) grouper and fish naturally infected with nodavirus. The myostatin gene comprises three exons and two introns and is transcribed as a 2778-bp mRNA length that encodes a 376-aa precursor protein. All exon-intron boundaries conformed to the consensus sequences. Alignment of the upstream sequences indicated that the grouper myostatin promoter has been highly conserved during evolution. Sequence analyses of 1936 bp of the upstream region revealed ten E-box motifs. The protein was consistent with the predicted molecular weight (approximately 42 kDa) of the unprocessed monomeric precursor protein and the processed myostatin form of the protein secreted into the plasma. Transient transfection studies revealed that the activity of the myostatin promoter decreased in a subset of viral titers. Grouper naturally infected with nodavirus displayed downregulation of the myostatin protein.

Original language	English
Pages (from-to)	215-227
Number of pages	13
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.cbd.2007.04.003
Publication status	Published - 2007 Sept

All Science Journal Classification (ASJC) codes

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Molecular Biology
Genetics

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10.1016/j.cbd.2007.04.003

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title = "Myostatin gene organization and nodavirus-influenced expression in orange-spotted grouper (Epinephelus coioides)",

abstract = "The relationship(s) between nodavirus infection and myostatin expression in the skeletal muscle tissue of grouper is unclear. To investigate, the grouper (Epinephelus coioides) myostatin gene was cloned and cDNA was utilized to examine the expression of the gene in skeletal muscle and serum of healthy (uninfected) grouper and fish naturally infected with nodavirus. The myostatin gene comprises three exons and two introns and is transcribed as a 2778-bp mRNA length that encodes a 376-aa precursor protein. All exon-intron boundaries conformed to the consensus sequences. Alignment of the upstream sequences indicated that the grouper myostatin promoter has been highly conserved during evolution. Sequence analyses of 1936 bp of the upstream region revealed ten E-box motifs. The protein was consistent with the predicted molecular weight (approximately 42 kDa) of the unprocessed monomeric precursor protein and the processed myostatin form of the protein secreted into the plasma. Transient transfection studies revealed that the activity of the myostatin promoter decreased in a subset of viral titers. Grouper naturally infected with nodavirus displayed downregulation of the myostatin protein.",

author = "Chen, {Y. M.} and Wei, {C. Y.} and Chien, {C. H.} and Chang, {H. W.} and Huang, {S. I.} and Yang, {H. L.} and Chen, {T. Y.}",

note = "Funding Information: We are grateful to Mr. Fu-Ping Chang and Mr. Nai-Heng Yu (Fish Breeding Association Taiwan) for critical comments on the manuscript. This research was supported by National Science Council (NSC93-2313-B-006-004), the National Science and Technology Program for Agricultural Biotechnology (NSC94-2317-B-006-004), and the plan of University Advancement, Ministry of Education, Taiwan.",

year = "2007",

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doi = "10.1016/j.cbd.2007.04.003",

language = "English",

volume = "2",

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AU - Chen, Y. M.

AU - Wei, C. Y.

AU - Chien, C. H.

AU - Chang, H. W.

AU - Huang, S. I.

AU - Yang, H. L.

AU - Chen, T. Y.

N1 - Funding Information: We are grateful to Mr. Fu-Ping Chang and Mr. Nai-Heng Yu (Fish Breeding Association Taiwan) for critical comments on the manuscript. This research was supported by National Science Council (NSC93-2313-B-006-004), the National Science and Technology Program for Agricultural Biotechnology (NSC94-2317-B-006-004), and the plan of University Advancement, Ministry of Education, Taiwan.

PY - 2007/9

Y1 - 2007/9

N2 - The relationship(s) between nodavirus infection and myostatin expression in the skeletal muscle tissue of grouper is unclear. To investigate, the grouper (Epinephelus coioides) myostatin gene was cloned and cDNA was utilized to examine the expression of the gene in skeletal muscle and serum of healthy (uninfected) grouper and fish naturally infected with nodavirus. The myostatin gene comprises three exons and two introns and is transcribed as a 2778-bp mRNA length that encodes a 376-aa precursor protein. All exon-intron boundaries conformed to the consensus sequences. Alignment of the upstream sequences indicated that the grouper myostatin promoter has been highly conserved during evolution. Sequence analyses of 1936 bp of the upstream region revealed ten E-box motifs. The protein was consistent with the predicted molecular weight (approximately 42 kDa) of the unprocessed monomeric precursor protein and the processed myostatin form of the protein secreted into the plasma. Transient transfection studies revealed that the activity of the myostatin promoter decreased in a subset of viral titers. Grouper naturally infected with nodavirus displayed downregulation of the myostatin protein.

AB - The relationship(s) between nodavirus infection and myostatin expression in the skeletal muscle tissue of grouper is unclear. To investigate, the grouper (Epinephelus coioides) myostatin gene was cloned and cDNA was utilized to examine the expression of the gene in skeletal muscle and serum of healthy (uninfected) grouper and fish naturally infected with nodavirus. The myostatin gene comprises three exons and two introns and is transcribed as a 2778-bp mRNA length that encodes a 376-aa precursor protein. All exon-intron boundaries conformed to the consensus sequences. Alignment of the upstream sequences indicated that the grouper myostatin promoter has been highly conserved during evolution. Sequence analyses of 1936 bp of the upstream region revealed ten E-box motifs. The protein was consistent with the predicted molecular weight (approximately 42 kDa) of the unprocessed monomeric precursor protein and the processed myostatin form of the protein secreted into the plasma. Transient transfection studies revealed that the activity of the myostatin promoter decreased in a subset of viral titers. Grouper naturally infected with nodavirus displayed downregulation of the myostatin protein.

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Myostatin gene organization and nodavirus-influenced expression in orange-spotted grouper (Epinephelus coioides)

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