@article{f25b50708cb445b78e0563c5cdafc558,
title = "N-α-Acetyltransferase 10 Protein Suppresses Cancer Cell Metastasis by Binding PIX Proteins and Inhibiting Cdc42/Rac1 Activity",
abstract = "N-α-acetyltransferase 10 protein, Naa10p, is an N-acetyltransferase known to be involved in cell cycle control. We found that Naa10p was expressed lower in varieties of malignancies with lymph node metastasis compared with non-lymph node metastasis. Higher Naa10p expression correlates the survival of lung cancer patients. Naa10p significantly suppressed migration, tumor growth, and metastasis independent of its enzymatic activity. Instead, Naa10p binds to the GIT-binding domain of PIX, thereby preventing the formation of the GIT-PIX-Paxillin complex, resulting in reduced intrinsic Cdc42/Rac1 activity and decreased cell migration. Forced expression of PIX in Naa10-transfected tumor cells restored the migration and metastasis ability. We suggest that Naa10p functions as a tumor metastasis suppressor by disrupting the migratory complex, PIX-GIT- Paxillin, in cancer cells.",
author = "Hua, {Kuo Tai} and Tan, {Ching Ting} and Gunnar Johansson and Lee, {Jang Ming} and Yang, {Pei Wen} and Lu, {Hsin Yi} and Chen, {Chi Kuan} and Su, {Jen Liang} and Chen, {Po Shen B.} and Wu, {Yu Ling} and Chi, {Chia Chun} and Kao, {Hsin Jung} and Shih, {Hou Jung} and Chen, {Min Wei} and Chien, {Ming Hsien} and Chen, {Pai Sheng} and Lee, {Wei Jiunn} and Cheng, {Tsu Yao} and George Rosenberger and Chai, {Chee Yin} and Yang, {Chih Jen} and Huang, {Ming Shyan} and Lai, {Tsung Ching} and Chou, {Teh Ying} and Michael Hsiao and Kuo, {Min Liang}",
note = "Funding Information: This work was supported by grants from the National Science Council, Taiwan (NSC97-2320-B-002-036). We thank Dr. Ivan de Curtis for providing HA-βPIX and deletion constructs, Dr. Richard A. Cerione and Dr. Qiyu Feng for providing Myc-αPIX, Myc-βPIX, and HA-PAK3, Dr. Johan R. Lillehaug and Dr. Thomas Arnesen for providing Naa10-V5, Naa11-V5, and Naa15-V5, Dr. Richard Premont for providing the Flag-GIT1 and Flag-GIT2 plasmids and antibodies for αPIX, Dr. Peter Hordijk for providing GST-βPIX, and Dr. Jonathan Chernoff for providing the Myc-PAK1 plasmid. We also thank Dr. Pan-Chyr Yang and Dr. Ruey-Hwa Chen for valuable discussions and helpful comments, Dr.Yung-Ming Jeng for assistance with pathological evaluation and Dr. Shuang-En Chuang for assistance with Naa10-R82A cloning. We thank the staff of the Second Core Lab, Department of Medical Research, National Taiwan University Hospital for technical support during the study. ",
year = "2011",
month = feb,
day = "15",
doi = "10.1016/j.ccr.2010.11.010",
language = "English",
volume = "19",
pages = "218--231",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "2",
}